| 利用微卫星分子标记研究我国16份披碱草遗传多样性 |
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| 引用本文： | 孙建萍,袁庆华.利用微卫星分子标记研究我国16份披碱草遗传多样性[J].草业科学,2006,23(8):40-44. |
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| 作者姓名： | 孙建萍 袁庆华 |
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| 作者单位： | 中国农业科学院畜牧研究所,北京,100094;上海市农业科学院生物技术中心,上海,201106;中国农业科学院畜牧研究所,北京,100094 |
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| 基金项目： | 国家科技部基础研究专项基金(2003-2005) |
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| 摘 要： | 利用微卫星(Microsatellite)分子标记对我国16份披碱草Elymus dahuricus进行遗传多样性研究。研究共筛选了38对引物,其中18对引物有扩增条带,占总数的47.4%,具有多态性并且扩增效果较好的引物有6对,占总数的15.8%。用筛选出的这6对引物对供试的16份材料进行微卫星分析,6对引物共检测出26个等位基因,平均每对引物所产生的平均等位基因数为4.3个位点;利用微卫星分子标记数据,对其居群遗传分化进行研究,结果表明:披碱草77.36%的遗传变异出现在居群内,因此,披碱草的遗传多样性主要存在于居群内;对16份披碱草进行聚类分析,构建了亲缘关系树状图,16份供试材料大致分为5类。
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| 关 键 词： | 微卫星 分子标记 披碱草 |
| 文章编号： | 1001-0629（2006）08-0040-05 |
| 收稿时间： | 07 7 2005 12:00AM |
| 修稿时间： | 2005-07-07 |
Study of the genetic diversity of sixteen accessions of Elymus dahuricus with microsatellite molecular markers |
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| SUN Jian-ping,YUAN Qing-hua.Study of the genetic diversity of sixteen accessions of Elymus dahuricus with microsatellite molecular markers[J].Pratacultural Science,2006,23(8):40-44. |
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| Authors: | SUN Jian-ping YUAN Qing-hua |
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| Institution: | 1. Institute of Animal Husbandry, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2. The Bio-Tech Center of Shanghai Agricultural Sciences Academy, Shanghai 201106, China |
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| Abstract: | Genetic diversity of 16 accessions were studied at microsatellite level.Thirty-eight primer pairs were used for polymorphic selection,with six(15.8%) primer pairs produced polymorphic products.A total of twenty-six bands amplified from six primer pairs were selected for microsatellite analysis.Primers consistently indicated that 77.36% of the variation existed within the groups of the 16 accessions of Elymus dahuricus;By cluster analysis with sum of squares for microsatellite showed that 16 accessions can be divided into 5 groups with different characteristics,which could afford reference in deeper research of E.dahuricus. |
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| Keywords: | microsatellite molecular markers Elymus dahuricus |
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