| 水牛卵母细胞冷冻保存初步研究 |
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| 引用本文： | 邵庆勇,杨红远,牛自兵,李卫娟,朱兰,吕春荣,洪琼花.水牛卵母细胞冷冻保存初步研究[J].中国草食动物,2011(6):12-16. |
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| 作者姓名： | 邵庆勇 杨红远 牛自兵 李卫娟 朱兰 吕春荣 洪琼花 |
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| 作者单位： | 云南省畜牧兽医科学院;云南省芒市畜牧兽医局 |
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| 基金项目： | 云南省省院省校科技合作计划(2006YX08);云南省技术创新人才培养项目(2009CI 108) |
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| 摘 要： | ①用EFS30、EFS40、EDFS30、EDFS40四种玻璃化冷冻液对MⅡ期水牛卵母细胞进行毒性试验,结果表明:试验组卵母细胞形态正常率与对照组均无显著性差异(P>0.05);对卵母细胞孤雌激活后EDFS30、EDFS40组的卵裂率与对照组(75.28%)及EFS30、EFS40组差异显著(P<0.05);利用4种冷冻保护剂采用OPS法冷冻保存MⅡ期水牛卵母细胞,其中以EDFS40作为冷冻液时,卵母细胞冷冻解冻后孤雌激活卵裂率最高,达31.60%;以EDFS40作为冷冻液,比较了GMP法和OPS法的冷冻效果,结果表明GMP法冷冻效果好于OPS法。②采用不同预处理时间和平衡时间使用细管法常规冷冻G V期卵母细胞,结果表明预处理5 min、平衡15min组的形态正常率和极体排出率相对较高,分别为72.73%、27.27%。
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| 关 键 词： | 水牛 卵母细胞 玻璃化 冷冻保存 |
Morphology and Developmental Competence of Buffalo(Bubalus bubalis) Oocytes:Effect of Vitrification and Slow Freezing |
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| Shao Qing-yong,Yang Hong-yuan,Hong Qiong-hua.Morphology and Developmental Competence of Buffalo(Bubalus bubalis) Oocytes:Effect of Vitrification and Slow Freezing[J].China Herbivores,2011(6):12-16. |
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| Authors: | Shao Qing-yong Yang Hong-yuan Hong Qiong-hua |
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| Institution: | ,et al(Yunnan Academy of Animal Science and Veterinary,Kunming 650224,China) |
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| Abstract: | This study was first employed to investigate the developmental potential of in vitro matured buffalo oocytes vitrified by open-pulled straw(OPS) and glass micropipette(GMP) methods.The oocytes were first pretreated in 10 % EG for 0.5 min and then 25 sec equilibration in EFS30(group 1) or EFS40(group 2),or in 10 %EG+10 %DMSO for 0.5 min and then 25 sec equilibration in EDFS30(group 3) or EDFS40(group 4) before they were subsequently diluted in 0.25 mol/L sucrose for 1 min and 0.15 mol/L sucrose for 5 min.After dilution,the morphologically normal rates of the oocytes in the 4 groups were similar to that of the control.After parthenogenetic activation,the cleavage rate of oocytes in group 3 and group 4 were significantly lower(P<0.05) than that in group 1 and group 2,which were similar to that of control(75.28%).After OPS vitrification and warming,the oocytes in group 4 showed the highest cleavage rate(31.60%).However,when the oocytes in group 4 were cryopreserved by GMP method,the cleavage rate were 45.63%.Secondly,the G V oocytes were cryopresered by slow freezing.After thawing,the morphologically normal rate and the polar body extrusion rate of the oocytes were significantly lower when compared with those of control. |
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| Keywords: | buffalo oocyte vitrification cryopreservation |
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