| 苹果茎沟病毒梨分离物外壳蛋白基因的克隆和序列分析 |
| |
| 引用本文： | 郑银英,洪霓,王国平,胡红菊.苹果茎沟病毒梨分离物外壳蛋白基因的克隆和序列分析[J].植物病理学报,2006,36(1):62-67. |
| |
| 作者姓名： | 郑银英 洪霓 王国平 胡红菊 |
| |
| 作者单位： | 1. 华中农业大学植物科学技术学院, 武汉 430070;2. 国家果树脱毒种质资源室内保存中心, 武汉 430070;3. 湖北省农科院果茶桑蚕研究所, 武汉 430209 |
| |
| 基金项目： | 中国科学院资助项目;国家科技攻关项目 |
| |
| 摘 要： | 选取生物学和血清学特性存在较大差异的苹果茎沟病毒(Apple stem grooving virus,ASGV)梨分离物P-6-1-17、P-4-1-69、P-L2和P-D-21,采用RT-PCR法对3'端进行扩增,所获扩增片段经序列测定,全长分别为1089nt(P-6-1-17、P-L2)和1069nt(P-4-1-69、P-D-21)。4个分离物的CP基因均由714nt组成,其核苷酸和推导编码蛋白氨基酸序列同源性分别为89.8%~96.4%和94.9%~97.9%。将我国4个分离物的CP基因与已报道ASGV分离物及分子变种进行序列同源性比较和系统进化树分析,结果表明我国ASGV分离物至少可分为2个组群,其中生物学表现明显不同于其它分离物的P-L2,其CP基因核苷酸序列显示较大的变异。
|
| 关 键 词： | 苹果茎沟病毒 梨分离物 CP基因 序列分析 变异 |
| 文章编号： | 0412-0914(2006)01-0062-06 |
| 修稿时间： | 2005年2月18日 |
Cloning and sequence analysis for the CP gene of Apple stem grooving virus from pears |
| |
| ZHENG Yin-ying,HONG Ni,WANG Guo-ping,HU Hong-ju.Cloning and sequence analysis for the CP gene of Apple stem grooving virus from pears[J].Acta Phytopathologica Sinica,2006,36(1):62-67. |
| |
| Authors: | ZHENG Yin-ying HONG Ni WANG Guo-ping HU Hong-ju |
| |
| Institution: | 1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;2. National Indoor Conservation Center of Virus-free Gemplasms of Fruit Crops, Wuhan 430070, China;3. Research Institute of Fruit, Tea and Silkworm-Mulberry, Hubei Academy of Agricultural Sciences, Wuhan 430209, China |
| |
| Abstract: | Four isolates of Apple stem grooving virus,P-6-1-17, P-4-1-69, P-L2 and P-D-21 from pears in China, which displayed different reactions in biological and serological tests, were studied for their molecular characteristics. RT-PCR products of 3'end of the four isolates were cloned and sequenced. The sizes of cloned fragments are 1089 nt from P-6-1-17 and P-L2,and 1069 nt from P-4-1-69 and P-D-21,respectively,which include the 714 nt CP-coding region. The similarities of nucleotide and deduced amino acid sequences of the CP genes among these isolates are 89.8% to 96.4% and 94. 9% to 97. 9%,respectively. The sequence data obtained in the study were compared with previously reported sequences of ASGV isolates and sequence variant species from different sources. Results suggested that isolates P-6-1-17,P4-1-69,P-L2 and P-D-21 should be divided into two different subgroups. P-L2 showed different reactions in biological test and its CP gene also has a relatively high variability. |
| |
| Keywords: | ASGV pear isolates CP gene sequence analysis variability |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
