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| 龙牙百合的植株再生与遗传转化 | |
| 引用本文： | 刘菊华,金志强,徐碧玉,郑思乡,刘志敏.龙牙百合的植株再生与遗传转化[J].分子植物育种,2003,1(4):465-474. |
| 作者姓名： | 刘菊华 金志强 徐碧玉 郑思乡 刘志敏 |
| 作者单位： | 1. 中国热带农业科学院生物技术研究所国家重点实验室,海口,571101 2. 云南农业大学,昆明,650201 3. 湖南农业大学,长沙,410128 |
| 摘 要： | 以龙牙百合鳞片叶切块为外植体，通过多种培养基的比较试验，得出MS 2，4-D 2mg／L 6-BA 0．2mg／L是诱导愈伤的最佳培养基，MS 6-BA 1mg／L NAA 0．05mg／L是不定芽诱导及伸长的适宜培养基。MS N_AA2mg／L是生根的最佳培养基。本试验已建立了适用于龙牙百合遗传转化的快速高频再生系统。用携带有几丁质酶基因和β—1、3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经PCR和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16．7％，基因枪法的转化率为50％，因此可能基因枪转化法更适于龙牙百合的遗传转化。 |
| 关 键 词： | 龙牙百合 植株再生 遗传转化 外植体 培养基 基因枪法 蔬菜 |
The Regeneration and Transformation of Longya Lilium |
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| Liu Juhua Jin Zhiqiang Xu Biyu Zheng Sixiang Liu Zhimin Chinese Academy of Tropical Agricultural Sciences,National Key Laboratory of Biotechnology Research Institute,Haikou, Yunan Agricultural University,Kunming, Hunan Agricultural University,Changsha, Corresponding author,zhiqiangjin@sohu com.The Regeneration and Transformation of Longya Lilium[J].Molecular Plant Breeding,2003,1(4):465-474. | |
| Authors: | Liu Juhua Jin Zhiqiang Xu Biyu Zheng Sixiang Liu Zhimin Chinese Academy of Tropical Agricultural Sciences National Key Laboratory of Biotechnology Research Institute Haikou Yunan Agricultural University Kunming Hunan Agricultural University Changsha Corresponding author zhiqiangjin@sohu com |
| Institution: | Liu Juhua 1 Jin Zhiqiang 1* Xu Biyu 1 Zheng Sixiang 2 Liu Zhimin 3 1 Chinese Academy of Tropical Agricultural Sciences,National Key Laboratory of Biotechnology Research Institute,Haikou,571101 2 Yunan Agricultural University,Kunming,650201 3 Hunan Agricultural University,Changsha,410128 * Corresponding author,zhiqiangjin@sohu com |
| Abstract: | The scale leaf of Longya Lilium( lilium spp )was taken as explant Through the comparism experiment of different media,we got the result:the combination of MS +2,4-D 2mg/L+6-BA 0 2mg/L was the optimum medium for the induction of calli;the combination of MS +6-BA 1mg/L +NAA0 05mg/L was the best medium for shoot differentiation and elongation;the medium of MS +NAA2mg/L was the best for adventitious root The engineering bacterium which carried both I-Chi and I-Glu cDNA was pCG-II Two methods of Agrobacterium mediated and gene gun were used to transformate Longya Lilium The results of PCR analysis and southern dot blotting hybridization demonstrated that the Chi and Glu cDNA has been intergrated into host genome At the same time,compared Agrobacterium mediated method with gene gun method,the transformation frequency of the former was 16 7%,while the latter was 50%,so gene gun transformation method was suitable for Longya Lilium |
| Keywords: | Longya Lilium Genetic transformation Agrobacterium mediated Gene gun |
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