| 土壤浸提液中重金属对发光杆菌T_3的毒性效应 |
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| 引用本文： | 李兴春,信晶,杜显元,李鱼.土壤浸提液中重金属对发光杆菌T_3的毒性效应[J].安徽农业科学,2011,39(35):21986-21989. |
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| 作者姓名： | 李兴春 信晶 杜显元 李鱼 |
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| 作者单位： | 1. 中国石油安全环保技术研究院,北京,100083
2. 华北电力大学能源与环境研究院,北京,102206 |
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| 基金项目： | 国家"十一五"科技支撑项目 |
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| 摘 要： | 通过测定掺杂Pb2＋、Zn2＋、Cd2＋、Cu2＋和Cr3＋的土壤浸提液中明亮发光杆菌T3的抑制率,研究重金属复合污染对明亮发光杆菌T3的生物毒性效应。按照重金属离子等效应浓度配比的原理,设计均匀试验,用逐步多元回归法构建土壤水浸提液中重金属（Pb2＋、Zn2＋、Cd2＋、Cu2＋和Cr3＋）浓度（Xi）与发光菌抑制率（Y）的响应关系：Y=（-39.596±15.811）-（6.633±0.802）×（-lgXCu）（-lgXZn）＋（59.466±8.731）×（-lgXCd）（-lgXCr）。该模型具有较好的拟合优度（F检验和t检验的P〈0.05）,模型校正后的决定系数（R2adj）为0.933。内部样本验证表明,发光菌抑制率的预测值与试验值相对偏差最大为8.6%,说明该模型具有较好的预测能力。机理分析显示,Cu2＋和Zn2＋交互体现为拮抗作用,Cd2＋和Cr3＋交互体现为协同作用,故在重金属复合污染控制方面,有必要进一步研究Cu2＋和Zn2＋拮抗作用对降低复合毒性的贡献。
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| 关 键 词： | 重金属 复合污染 明亮发光杆菌T3 生物毒性 土壤浸提液 |
Toxicity Effects of Extraction Solutions of Soil Doped with Heavy Metals to Photobacterium phosphoreum T3 |
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| Institution: | LI Xing-chun et al(Research Institute of Safety and Environment Technology,China National Petroleum Corporation,Beijing 100083) |
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| Abstract: | Toxicity effects of composite pollution of heavy metals to Photobacterium phosphoreum were studied through determining the inhibition rate of Photobacterium phosphoreum in the extraction solutions of soil doped with Pb2+,Zn2+,Cd2+,Cu2+ and Cr3+.The response relation Y=(-39.596±15.811)-(6.633±0.802)×(-lgXCu)(-lgXZn) +(59.466±8.731)×(-lgXCd)(-lgXCr)] was developed between concentration of Pb2+,Zn2+,Cd2+,Cu2+ and Cr3+ in extraction solutions of soil and the inhibition rate of Photobacterium phosphoreum based on the uniform experimental design,with the principle of equivalent-effect concentration ratio method and the multiple stepwise regression.The model had significant goodness-of-fit with advantages in F-test and t-test(P<0.05) and a high adjusted correlation coefficient(R2adj=0.933).The results in the internal sample validation indicated that the maximum of relative deviation between experimental and calculated values of the inhibition rate was 8.6%.The mechanism analysis showed that the interaction between Cu2+ and Zn2+ was antagonistic effect;while the interaction between Cd2+ and Cr3+ was synergistic effect.The findings of this study underlined the necessity of further research on the antagonistic effect between Cu2+ and Zn2+ to reduce composite toxicity in heavy metal pollution control. |
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| Keywords: | Heavy metals Composite pollution Photobacterium phosphoreum T3 Biotoxicity Extraction solutions of soil |
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