| 基于生物技术调控棘胸蛙繁殖与养殖的研究 |
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| 引用本文： | 刘韬,庄志鸿,杨声强.基于生物技术调控棘胸蛙繁殖与养殖的研究[J].中国农学通报,2009,25(7):26-29. |
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| 作者姓名： | 刘韬 庄志鸿 杨声强 |
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| 作者单位： | 1. 福建省农科院生物技术研究所,福州,350003
2. 屏南县清水源棘胸蛙养殖有限公司,福建屏南,350923 |
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| 摘 要： | 【研究目的】通过生物技术调控棘胸蛙繁殖，可提前并能集中产卵，达到同步孵化、批量出苗的目的。【方法】采用生殖激素绒毛膜促性腺激素（HCG）和促黄体素释放激素A3（LRH-A3）对棘胸蛙进行人工催产作用。【结果】HCG对棘胸蛙无催产作用，LRH-A3有明显的催产作用但授精不完全，而二者的组合处理不但催产作用明显，而且授精完全。在4~6月用HCG+LRH-A3激素组合催产后，棘胸蛙卵至变态为幼蛙的时间为73~89 d，均在9月以前完成由蝌蚪至幼蛙的变态。【结论】用HCG+LRH-A3激素组合对棘胸蛙进行生物调控，可以在实际生产中推广应用。
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| 关 键 词： | 绿芦笋 绿芦笋 育苗技术 田间管理 设施栽培 标准化栽培 |
| 收稿时间： | 2008-12-31 |
| 修稿时间： | 2009-02-05 |
Artificial Control on Reproduction and Breeding of Rana spinosa David based on Biotechnology |
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| Liu Tao,Zhuang Zhihong,Yang Shengqiang.Artificial Control on Reproduction and Breeding of Rana spinosa David based on Biotechnology[J].Chinese Agricultural Science Bulletin,2009,25(7):26-29. |
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| Authors: | Liu Tao Zhuang Zhihong Yang Shengqiang |
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| Institution: | Liu Tao, Zhuang Zhihong, Yang Shengqiang (1. Biotechnology Institute, Fujian Academy of Agricultural ,Sciences, Fuzhou 350003; 2.Piangnan Qingshui Rana spinosa Breeding Corporation, Pingnan Fujian 350923) |
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| Abstract: | 【OBJECTIVE】The reproduction of Rana spinosa David was artificially controlled by using biotechnology, in order to advance and centralize the ovulation, synchronize incubation and batch breeding of tadpole.【METHOD】HCG and LRH-A3 were used to make artificial inducing spawning of R. spinosa【RESULTS】No inducing effect of HCG was found on spawning of R. spinosa. LRH-A3 had obvious effect on spawning, but the insemination of R. spinosa was inadequacy. The combination of HCG and LRH-A3 not only had significant function on spawning, but also made complete insemination. After treated with HCG+LRH-A3 in April to June, the growth time from spawn to young frog was 73-89 d, all before September. 【CONCLUSION】The combination of HCG and LRH-A3 could be used to artificial control R. spinosa in practical breeding. |
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| Keywords: | Rana spinosa bioteehnology reproduction breeding artificial control |
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