| 从动物毛中提取DNA研究初探 |
| |
| 引用本文： | 郑冬,孔瑾.从动物毛中提取DNA研究初探[J].野生动物,1996(2):24-26. |
| |
| 作者姓名： | 郑冬 孔瑾 |
| |
| 作者单位： | 东北林业大学 |
| |
| 摘 要： | 利用蛋白酶Ｋ分解毛发蛋白，并使用酚和氧仿除去蛋白质杂质方法提取毛中ＤＮＡ。结果测量获得的数据和曲线表明：动物脱落毛发以及从保存多年标本获得的毛发均可用来提取到具有一定纯度和数量的ＤＮＡ，完全可以满足聚合酶链式反应（ＰＣＲ）所需。
|
| 关 键 词： | 聚合酶链式反应（PCR），脱落毛发，DNA提取 |
Study on DNA Typing from Animal Hairs |
| |
| Authors: | Zheng Dong Kong Jin Shi Shaoye |
| |
| Abstract: | Data and figures show that DNA with certains purity and quantity could be recovered enough for Polpoerase Chain Reaction(PCR)from both animal shed hairs and hairs ofspeciments kept for many years, which would be digested with proteinase K followed by extraction phenol and chloroform. |
| |
| Keywords: | PCR Shed hair DNA extraction |
| 本文献已被 CNKI 维普 等数据库收录！ |
