| 玉米耐盐基因ZmHKT1;5在烟草中的功能验证 |
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| 引用本文： | 姜志磊,刘艳芝,韦正乙,姜昱,金峰学,李毅丹.玉米耐盐基因ZmHKT1;5在烟草中的功能验证[J].玉米科学,2017,25(5):32-39. |
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| 作者姓名： | 姜志磊 刘艳芝 韦正乙 姜昱 金峰学 李毅丹 |
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| 作者单位： | 吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033,吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室, 长春 130033 |
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| 基金项目： | 国家自然科学基金（31100242） |
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| 摘 要： | HKT类基因是与植物耐盐性密切相关的一类基因。在作物中HKT蛋白可通过排出Na+来维持植物体内的Na~+/K~+平衡,从而影响植物耐盐性。通过在烟草中过表达玉米ZmHKT1;5基因,验证该基因具有提高植物耐盐性的作用。结果表明,过表达ZmHKT1;5基因的T0代材料即显示出叶片耐盐能力的明显提高;T2代转基因株系种子在含盐培养基上的发芽能力明显强于野生型材料,T2代转基因株系幼苗阶段的耐盐能力也得到了明显的提高。通过比较在盐胁迫后2月龄的转基因材料和野生型材料的生理指标,发现野生型材料中MDA和H_2O_2的含量相较转基因材料发生了更为明显的上升,说明转基因材料中过表达ZmHKT1;5基因有效降低了盐胁迫引起的过氧化物积累。综合转基因验证的结果,证明ZmHKT1;5基因具有提高植物耐盐性的作用。
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| 关 键 词： | 玉米 ZmHKT1; 5基因 转基因 盐胁迫 |
| 收稿时间： | 2017/4/12 0:00:00 |
Overexpression of ZmHKT1;5 Enhances Tolerance to Salt Stress in Nicotiana tabacum |
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| JIANG Zhi-lei,LIU Yan-zhi,WEI Zheng-yi,JIANG Yu,JIN Feng-xue and LI Yi-dan.Overexpression of ZmHKT1;5 Enhances Tolerance to Salt Stress in Nicotiana tabacum[J].Journal of Maize Sciences,2017,25(5):32-39. |
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| Authors: | JIANG Zhi-lei LIU Yan-zhi WEI Zheng-yi JIANG Yu JIN Feng-xue and LI Yi-dan |
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| Institution: | Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, China,Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, China,Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, China,Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, China,Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, China and Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, China |
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| Abstract: | The HKT like genes are closely related to salt tolerance in plants. HKT proteins can affect the salt tolerance by discharging Na+ to maintain Na+/K+ balance. In this study, the maize ZmHKT1;5 gene was overexpressed in tobacco(Nicotiana tabacum) to verify that the gene also had the effect of improving plant salt tolerance. The results showed that the T0 leaves of overexpressing ZmHKT1;5 showed a significant increase in salt tolerance. The germination ability of T2 seeds was much better than that of wild-type materials under salt stress. The results also showed that the content of MDA and H2O2 in the wild-type materials were higher than those in the transgenic, which indicated that overexpressing ZmHKT1;5 gene effectively reduced the peroxide accumulation caused by salt stress in the transgenic materials. Based on the results of our research, it was proved that the ZmHKT1;5 gene could improve the salt tolerance in plants. |
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| Keywords: | Maize ZmHKT1; 5 Transgenic Salt tolerance |
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