| 大豆体细胞胚胎发生系统组织学研究 |
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| 引用本文： | 赵桂兰,杨向东,郭东全,胡赞民.大豆体细胞胚胎发生系统组织学研究[J].农业科学与技术,2008,9(2):49-53. |
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| 作者姓名： | 赵桂兰 杨向东 郭东全 胡赞民 |
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| 作者单位： | [1]吉林省农业科学院生物技术研究中心,长春130124 [2]中国科学院遗传与发育研究所,北京100101 |
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| 基金项目： | 国家自然科学基金，吉林省科技发展计划，国家高技术研究发展计划(863计划) |
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| 摘 要： | 通过对栽培大豆品种体细胞胚胎发生频率组织学进行研究，了解在农杆菌和卡那霉素存在的条件下，大豆未成熟子叶胚性细胞的发生、分化、发育过程和胚胎发生规律，为大豆未成熟子叶诱导体细胞胚发生高效转化体系提供理论依据。
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| 关 键 词： | 大豆 体细胞 胚胎发生 系统组织学 子叶胚性细胞 |
Histological Study on Soybean Somatic Embryogenesis |
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| ZHA Gui-lan,YANG Xiang-dong,GU Dong-quan,HU Zan-min.Histological Study on Soybean Somatic Embryogenesis[J].Agricultural Science ＆ Technology,2008,9(2):49-53. |
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| Authors: | ZHA Gui-lan YANG Xiang-dong GU Dong-quan HU Zan-min |
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| Institution: | ZHAO Gui-lan ,YANG Xiang-dong,GUO Dong-quan, HU Zan-min( 1. Biotechnology Research Center,Jilin Academy of Agricultural Sciences, Changchun 130124; 2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101) |
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| Abstract: | Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryo-genesis system of soybean was used to conduct genetic transformation of soybean because of its several advantages such as higher transformational efficiency, beetter synchronism and fewer plant chimeras among transgenic plants. After infected with agrobacterium tumefaciens,the initiation, differentiation and de-velopment of young cotyledon embryogenic cell of soybean which was cultured on selective culture medium with kanamycin were investigated through histo-logical study. The resuh showed that somatic embryo was differentiated in non-bud differentiation way. The embryogenic coils were differentiated from epi-dermis of explant or cells in 1 layer or 2 layers, with the division of embryogenic cells and degradation and disorganization of surrounding cells, the embryo-genic cells would form embryoid with analogous suspensor structure, later, globular embryoid would extrude from epidermis then developed into heart-shape embryo. The experiment was expected to provide theoretical reference for the construction of high transformational system of using plant somatic embryogene-sis induced by young cotyledon of soybean. |
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| Keywords: | Soybean Somatic embryagenesis Histology |
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