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| HPMCP微胶囊蛋白质包裹量的测定方法 | |
| 引用本文： | 张明政,刘波,林乃铨,阮传清.HPMCP微胶囊蛋白质包裹量的测定方法[J].中国农学通报,2009,25(5):32-37. |
| 作者姓名： | 张明政 刘波 林乃铨 阮传清 |
| 作者单位： | 1. 福建省农业科学院农业生物资源研究所,福州,350003;福建农林大学植物保护学院,福州,350002 2. 福建省农业科学院农业生物资源研究所,福州,350003 3. 福建农林大学植物保护学院,福州,350002 |
| 基金项目： | 福建省财政专项福建省农业科学院科技创新团队建设基金，国家高技术研究发展计划(863计划) |
| 摘 要： | 本文采用复相乳液法制备HPMCP的BSA微胶囊，研究常用蛋白质定量方法考马斯亮蓝染色法、福林-酚法、紫外吸收法对测定HPMCP微胶囊蛋白包裹量受壁材、乳化剂、亲水性保护胶体的影响。结果得到制备的微胶囊呈圆形或椭圆形，没有粘结在一起的现象，三种方法中福林-酚法影响程度最小，适合测定HPMCP蛋白微胶囊的包裹量。 |
| 关 键 词： | 自主创新 自主创新 福建 粮食产业化 措施 |
| 收稿时间： | 2008-12-03 |
| 修稿时间： | 2009-01-22 |
Study of using different means to mensurate envelopment rate of protein of HPMCP microcapsuals |
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| Zhang Mingzheng,Liu Bo,Lin Naiquan,Ruan Chuanqing.Study of using different means to mensurate envelopment rate of protein of HPMCP microcapsuals[J].Chinese Agricultural Science Bulletin,2009,25(5):32-37. | |
| Authors: | Zhang Mingzheng Liu Bo Lin Naiquan Ruan Chuanqing |
| Institution: | Zhang Mingzheng, Liu Bo, Lin Naiquan, Ruan Chuanqing (1.Institute of Agro-Biological Resources, Fujian Academy of Agricultural Sciences, Fuzhou 350003; 2College of Plant Protection, Fufian Agriculture and Forestry University, Fuzhou 350002) |
| Abstract: | This paper studied the means of diphase emulsion to make bovine albumin microcapsuals with hydroxy proprl methyl celluloxe phthalate, effection of the wrap material, reagent of emulsification, hydrophilic colloid of protection and pH of testing protein on the bradford protein assay, the folin-phenol assay and UV-absorbing methods for testing the envelopment protein capacity of microcapsules. The end that shape of microcapsules was orbicular or elliptic and no conglutination, the best means for testing envelopment protein capacity of microcapsules is the Folin-pHenol assay because it has the least effection. |
| Keywords: | hydroxy proprl methyl celluloxe phthalate microcapsuals envelopment quantum coomassie blueStaining Solution folin-phenol reagent |
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