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菠菜离体叶绿体培养研究
引用本文:程霞英,杨宗岐.菠菜离体叶绿体培养研究[J].安徽农业科学,2007,35(15):4419-4419,4464.
作者姓名:程霞英  杨宗岐
作者单位:廊坊师范学院生命科学院,河北廊坊,065000;浙江理工大学生物工程研究所,浙江杭州,310018
基金项目:河北省廊坊师范学院院级基金资助项目(LS200506)
摘    要:研究了不同pH值、温度、光照、悬浮介质对菠菜离体叶绿体存活时间的影响,结果表明,在pH为7.6、温度为5℃(低温)的黑暗条件下用悬浮介质Ⅰ培养的菠菜离体叶绿体活性保持时间较长。

关 键 词:离体叶绿体  培养  菠菜
文章编号:0517-6611(2007)15-04419-01
修稿时间:2007-03-18

Study on the Spinach Chloroplast Culture in Vitro
CHENG Xia-ying, et al.Study on the Spinach Chloroplast Culture in Vitro[J].Journal of Anhui Agricultural Sciences,2007,35(15):4419-4419,4464.
Authors:CHENG Xia-ying  
Institution:School of Life Sience,Langfang Normal College,Langfang, Hebei 065000
Abstract:The influence of different entironment factor such as pH,temperature,illumination and the suspending medium on the spinach(Spinacia oleracea L.) chloroplast culture in vitro was studied.The results showed that there was longest living time of isolated chloroplast in the condition of pH 7.6,temperature 5 ℃,darkness and suspending medium I.
Keywords:Isolated chloroplast  Culture  Spinach
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