| 罗汉果皂苷对肥胖小鼠脂代谢的改善作用研究 |
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| 引用本文： | 梁业飞,黄盼玲,李叔惠,黄国栋,夏星,李宇清.罗汉果皂苷对肥胖小鼠脂代谢的改善作用研究[J].中国畜牧兽医,2022,49(6):2129-2136. |
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| 作者姓名： | 梁业飞 黄盼玲 李叔惠 黄国栋 夏星 李宇清 |
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| 作者单位： | 1. 广西中医药大学, 广西高校中药药理重点实验室, 南宁 530200;2. 广西中医药大学公共卫生与管理学院, 南宁 530200;3. 钦州市第一人民医院临床药学科, 钦州 535000 |
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| 基金项目： | 国家自然科学基金项目（81960728）；中药学广西一流学科（桂教科研[2022]1号）；广西第八批特聘专家项目（壮瑶药质量标准研究（桂人才通字[2019]13号）；广西中医药大学一流学科开放课题（2018XK030） |
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| 摘 要： | 【目的】 探讨罗汉果皂苷改善高脂喂养诱发肥胖的小鼠脂代谢异常的作用，并探讨其作用机制。【方法】 选取健康雄性昆明小鼠60只，以高脂饲料喂养4周后，随机分为6组：空白组、模型组、辛伐他汀组，以及罗汉果皂苷高（200 mg/（kg·d））、中（100 mg/（kg·d））、低（50 mg/（kg·d））剂量组，连续灌胃给药4周，同时维持高脂喂养。给药完成后，称量各组小鼠体重及脂肪组织重量，计算脂肪系数；检测小鼠血清和肝脏组织甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）及低密度脂蛋白胆固醇（LDL-C）的含量，以及血清载脂蛋白A1（ApoA1）和载脂蛋白B （ApoB）含量，并测定肝脏中脂蛋白脂肪酶（LPL）和肝酯酶（HL）的活性。【结果】 与空白组相比，模型组小鼠体重、脂肪重量及脂肪系数均极显著升高（P<0.01）。与模型组相比，罗汉果皂苷高、中剂量组小鼠的脂肪重量及脂肪系数均显著降低（P<0.05）；高剂量罗汉果皂苷显著或极显著降低了小鼠血清及肝脏TC、LDL-C含量（P<0.05；P<0.01），极显著升高了血清HDL-C含量（P<0.01）；高、中剂量罗汉果皂苷极显著降低了血清ApoB含量（P<0.01），极显著或显著升高了小鼠肝脏LPL和HL的活性（P<0.01；P<0.05）。【结论】 罗汉果皂苷能预防高脂饲料诱导的肥胖小鼠血脂、肝脂和体脂的增加，其可能的作用机制是通过影响ApoB的合成及HL、LPL的活性。研究结果为将罗汉果皂苷开发为新的减肥降脂产品提供了依据。
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| 关 键 词： | 罗汉果皂苷 高脂血症 脂代谢 肥胖 |
| 收稿时间： | 2021-12-27 |
Effect of Mogrosides on Lipid Metabolism in Obese Mice |
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| LIANG Yefei,HUANG Panling,LI Shuhui,HUANG Guodong,XIA Xing,LI Yuqing.Effect of Mogrosides on Lipid Metabolism in Obese Mice[J].China Animal Husbandry & Veterinary Medicine,2022,49(6):2129-2136. |
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| Authors: | LIANG Yefei HUANG Panling LI Shuhui HUANG Guodong XIA Xing LI Yuqing |
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| Institution: | 1. Key Laboratory of TCM Pharmacology of Guangxi University of Chinese Medicine-Education Department of Guangxi Zhuang Autonomous Region, Guangxi University of Chinese Medicine, Nanning 530200, China;2. College of Public Health and Management, Guangxi University of Chinese Medicine, Nanning 530200, China;3. Department of Clinical Pharmacy, Qinzhou First People's Hospital, Qinzhou 535000, China |
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| Abstract: | 【Objective】 This study was aimed to investigate the effects of mogrosides on improving the lipid metabolism abnormality in obese mice caused by high-fat diet, and explore its mechanism of action.【Method】 60 healthy male Kunming mice were randomly divided into 6 groups after being fed with high-fat diet for 4 weeks, including normal group, model group, simvastatin group, and high (200 mg/(kg·d)), medium (100 mg/(kg·d)) and low (50 mg/(kg·d)) dose mogrosides groups.The mogrosides dose groups were administered by gavage for 4 weeks while maintaining high-fat feeding.After the administration was completed, the body weight of mice in each group and the weight of adipose tissue were obained, and the fat coefficient was calculated.The contents of triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) in serum and liver, the contents of apolipoprotein A1 (ApoA1) and apolipoprotein B (ApoB) in serum, and the activities of lipoprotein lipase (LPL) and hepatic esterase (HL) in liver were measured.【Result】 Compared with blank group, the body weight, fat weight and fat coefficient of mice in model group were extremely significantly increased (P<0.01).Compared with model group, the fat weight and fat coefficient of mice in high and medium dose mogrosides groups were significantly reduced (P<0.05);The contents of TC and LDL-C in serum and liver of mice in high dose mogrosides group were significantly or extremely significantly reduced (P<0.05 or P<0.01), and the content of HDL-C in serum was extremely significantly increased (P<0.01);The content of ApoB in serum of mice in high and medium doses mogrosides groups was extremely significantly reduced (P<0.01), and the activities of LPL and HL in liver of mice were extremely significantly or significantly increased (P<0.01 or P<0.05).【Conclusion】 The mogrosides could prevent the increase of blood lipid, liver fat and body fat in obese mice induced by high-fat diet, its possible mechanism was by affecting the synthesis of ApoB and the activities of HL and LPL.The results provided a reference for the development of the mogrosides as a potential weight lowering and lipid lowering agent. |
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| Keywords: | mogrosides hyperlipidemia lipid metabolism obesity |
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