| 副猪嗜血杆菌血清7型菌株毒力及耐药性分析 |
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| 引用本文： | 王治方,徐引弟,朱文豪,张青娴,焦文强,李海利,王克领.副猪嗜血杆菌血清7型菌株毒力及耐药性分析[J].中国畜牧兽医,2022,49(8):3245-3255. |
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| 作者姓名： | 王治方 徐引弟 朱文豪 张青娴 焦文强 李海利 王克领 |
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| 作者单位： | 河南省农业科学院畜牧兽医研究所, 郑州 450002 |
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| 基金项目： | 国家重点研发计划项目(2021YFD1301205);河南省重点科技攻关项目(212102110378);河南省农业科学院自主创新项目(2022ZC43) |
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| 摘 要： | 【目的】了解河南地区副猪嗜血杆菌(Haemophilus parasuis,Hps)血清7型流行菌株的毒力及耐药情况。【方法】以Hps血清7型参考菌株为对照,对6株Hps血清7型临床分离菌株进行16S rRNA基因扩增、序列分析、毒力基因检测、致病性试验和药敏试验研究。【结果】16S rRNA基因测序及相似性比对结果显示,菌株1436与参考菌株0007相似性为100%,菌株1565、1624与参考菌株0007相似性均为98.6%。系统发育树分析表明,菌株1436与参考菌株0007亲缘关系最近;菌株1624与参考菌株0007亲缘关系最远。6株临床菌株与参考菌株表现为2种毒力基因型,菌株均携带有vta1、vta2、vta3、wza、nanH、cdtA、cdtB、cdtC和espP2毒力基因,其中5株临床菌株还携带ompP2毒力基因。豚鼠致病性试验结果显示,临床菌株毒力较参考菌株表现出不同程度的增强,参考菌株不能感染豚鼠发病,未表现任何临床症状;临床菌株可感染豚鼠发病,表现出一定的临床症状,发病率为20%~40%,死亡率为0。参考菌株和临床菌株均对头孢他啶和头孢噻呋敏感,对强力霉素、氟苯尼考中介,对新霉素、卡那霉素、阿米卡星、红霉素、替米考星等耐药,且均表现出多重耐药现象。【结论】Hps血清7型临床菌株有一定的致病性,但毒力较弱,对头孢类药物敏感,有明显的多重耐药现象,临床上应注意对该血清型的防控。本研究为Hps血清7型流行病学、致病机制研究奠定了基础,为Hps血清7型的临床防控提供了参考依据。
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| 关 键 词： | 副猪嗜血杆菌(Hps) 血清7型 分型鉴定 毒力基因 致病性试验 药敏试验 |
| 收稿时间： | 2022-02-10 |
Analysis of Virulence and Drug Resistance of Haemophilus parasuis Serotype 7 Strains |
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| WANG Zhifang,XU Yindi,ZHU Wenhao,ZHANG Qingxian,JIAO Wenqiang,LI Haili,WANG Keling.Analysis of Virulence and Drug Resistance of Haemophilus parasuis Serotype 7 Strains[J].China Animal Husbandry & Veterinary Medicine,2022,49(8):3245-3255. |
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| Authors: | WANG Zhifang XU Yindi ZHU Wenhao ZHANG Qingxian JIAO Wenqiang LI Haili WANG Keling |
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| Institution: | Institute of Animal Husbandry and Veterinary Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China |
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| Abstract: | 【Objective】 This study was aimed to understand the virulence and drug resistance of Haemophilus parasuis (Hps) serotype 7 epidemic strains in Henan province.【Method】 Taking the reference strain of Hps serotype 7 as the control,the 16S rRNA gene amplification,sequence analysis,virulence gene detection,pathogenicity test and drug sensitivity test were carried out on 6 strains of Hps serotype 7 isolated and identified from clinical cases.【Result】 16S rRNA gene sequencing and similarity comparison showed that the similarity between strain 1436 and standard strain 0007 was 100%,and that between strain 1565 and 1624 and standard strain were 98.6%. Phylogenetic tree analysis showed that 1436 had the closest genetic relationship with standard strain 0007,1624 was the farthest related to standard strain 0007.Six clinical strains and standard strain showed two virulence genotypes.All strains carried vta1,vta2, vta3,wza,nanH,cdtA,cdtB,cdtC and espP2 virulence genes,and five clinical strains also carried ompP2 virulence gene.In the guinea pig pathogenicity test,the clinical strain showed different degrees of virulence enhancement compared with the reference strain.The reference strain could not infect guinea pigs and did not show any clinical symptoms.The clinical isolates could infect guinea pigs,showing some clinical symptoms,the incidence rates were 20% to 40%,and the mortality rate was 0.The standard strain and clinical strains were sensitive to ceftazidime and ceftiofur,they were mediated to doxycycline and florfenicolo,the resistance to antibiotics such as neomycin,kanamycin,amikacin,erythromycin and tilmicosin was strong,and all strains showed multiple drug resistance.【Conclusion】 Hps serotype 7 clinical strains had certain pathogenicity,but their virulence was weak,they were sensitive to cephalosporins and had obvious multi drug resistance.Clinically,the prevention and control of this serotype should be paid attention.This study laid a foundation for the epidemiology and pathogenesis of Hps serotype 7,and provided a reference basis for the clinical prevention and control of Hps serotype 7. |
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| Keywords: | Haemophilus parasuis (Hps) serotype 7 typing and identification virulence genes pathogenicity test drug sensitivity test |
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