| 梅花鹿成纤维细胞因子受体2基因多态性及其与茸重性状的关联分析 |
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| 引用本文： | 周雅,张禾垟,刘琳玲,李浩东,郑军军,王桂武.梅花鹿成纤维细胞因子受体2基因多态性及其与茸重性状的关联分析[J].中国畜牧兽医,2022,49(8):3006-3014. |
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| 作者姓名： | 周雅 张禾垟 刘琳玲 李浩东 郑军军 王桂武 |
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| 作者单位： | 中国农业科学院特产研究所, 长春 130112 |
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| 基金项目： | 中国农业科学院科技创新工程项目(CAAX-ASTIP-2016-USAOS02) |
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| 摘 要： | 【目的】探索梅花鹿成纤维细胞因子受体2(fibroblast growth factor receptor 2,FGFR2)基因多态性及其对茸重性状的影响。【方法】应用直接测序法对梅花鹿FGFR2基因的全部外显子进行测序分析,通过MassARRAY® SNP分型技术对314头24月龄梅花鹿进行基因分型和单倍型分析,分析FGFR2基因不同基因型和单倍型与茸重的关联性。【结果】在梅花鹿FGFR2基因中共发现12个多态性位点,其中5个位于外显子区域,且突变均未引起氨基酸改变,属于同义突变,其余7个位点均存在于内含子区域。分型结果显示,g.80975864 T>G位点未分型成功,后续对其余11个位点进行了分析,g.80943673 T>C、g.80943683 C>A及g.80938352 C>T 3个位点属于中度多态位点(0.25＜P＜0.5),其余位点均属于低度多态位点(P＜0.25)。χ2检验结果表明,g.80998742 G>A和g.80987708 G>A 2个位点偏离Hardy-Weinberg平衡(P＜0.05),其他9个位点均处于Hardy-Weinberg平衡(P＞0.05)。关联分析结果表明,11个多态性位点各基因型之间的茸重差异均不显著(P＞0.05)。单倍型结果显示,FGFR2基因存在5种单倍型,不同单倍型间梅花鹿茸重差异均不显著(P＞0.05)。【结论】FGFR2基因的11个突变位点可能不是影响梅花鹿茸重性状的关键位点。
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| 关 键 词： | 梅花鹿 成纤维细胞因子受体2(FGFR2)基因 SNP 鹿茸重量 |
| 收稿时间： | 2021-12-29 |
Analysis of FGFR2 Gene Polymorphism and Their Correlation with Antler Weight Traits in Sika Deer |
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| ZHOU Ya,ZHANG Heyang,LIU Linling,LI Haodong,ZHENG Junjun,WANG Guiwu.Analysis of FGFR2 Gene Polymorphism and Their Correlation with Antler Weight Traits in Sika Deer[J].China Animal Husbandry & Veterinary Medicine,2022,49(8):3006-3014. |
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| Authors: | ZHOU Ya ZHANG Heyang LIU Linling LI Haodong ZHENG Junjun WANG Guiwu |
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| Institution: | Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China |
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| Abstract: | 【Objective】 This study was performed to explore the polymorphism of fibroblast growth factor receptor 2 (FGFR2) gene in sika deer and its effect on antler weight traits.【Method】 All exons of FGFR2 gene of sika deer were sequenced and analyzed by direct sequencing.314 24-month-old sika deer were genotyped and haplotyped by MassARRAY® SNP typing technology,and the correlation between different genotypes and haplotypes of FGFR2 gene and antler weight traits was analyzed.【Result】 A total of 12 SNPs were found in the FGFR2 gene in sika deer,of which 5 were located in the exon region,and none of the mutations caused amino acid changes,belonging to synonymous mutations,and the other 7 SNPs were located in the intron region.The typing results showed that g.80975864 T>G site was not successfully typing,and the remaining 11 SNPs were subjected to subsequent analysis.The 3 SNPs of g.80943673 T>C,g.80943683 C>A and g.80938352 C>T belonged to moderately polymorphic (0.25<PIC<0.5),and the rest belonged to low-polymorphism (PIC<0.25). χ2 fitness test results showed that the 2 SNPs of g.80998742 G>A and g.80987708 G>A deviated from Hardy-Weinberg equilibrium (P<0.05),and the others were in Hardy-Weinberg equilibrium.(P>0.05).The results of association analysis showed that there was no significant difference in antler weight among the genotypes of the 11 SNPs (P>0.05).Haplotype results showed that there were five haplotypes in FGFR2 gene,and there was no significant difference between different haplotypes and antler weights (P>0.05).【Conclusion】 The 11 loci at FGFR2 were not the potential markers for antler weight. |
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| Keywords: | sika deer fibroblast growth factor receptor 2 (FGFR2) gene SNP antler weight |
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