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In vitro propagation of a medicinal plant: Tripterygium wilfordii Hook f. |
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| Authors: | Bi-hu? Chen |
| Institution: | (1) Forestry Biotechnology Research and Development Center, Fujian Academy of Forestry Sciences, Fuzhou, 350012, P. R. China;(2) Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, State Forestry Administration, Fuzhou, 350012, P. R. China |
| Abstract: | In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP (2.0 mg·L−1) and NAA (0.1 mg·L−1). The optimal multiplication medium was a modified MS medium supplemented with BAP (1.0 mg·L−1) and NAA (0.1 mg·L−1). This yielded a multiplication rate of 2.4 for each subculture. Slightly more than 92% of shoots rooted when cultured on a modified MS medium containing IBA (0.2 mg·L−1) and activated charcoal (0.5 mg·L−1). Activated charcoal promoted both a strong and a high rooting rate during the rooting phase. Plantlets were transferred to pots for a short acclimatization stage in a greenhouse where 95% of the plantlets survived. This highly reproducible procedure can be adopted for large-scale propagation of T. wilfordii. |
| Keywords: | in vitro propagation Tripterygium wilfordii Hook f multiplication rooting activated charcoal |
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