| 池塘和稻田两种养殖模式下建鲤肠道菌群、免疫酶活性及肌肉氨基酸比较分析 |
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| 引用本文： | 赵柳兰,龙亚男,罗杰,刘巧,周剑,杜军,周亚,杨佰维,杨淞.池塘和稻田两种养殖模式下建鲤肠道菌群、免疫酶活性及肌肉氨基酸比较分析[J].中国水产科学,2021,28(1):48-56. |
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| 作者姓名： | 赵柳兰 龙亚男 罗杰 刘巧 周剑 杜军 周亚 杨佰维 杨淞 |
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| 作者单位： | 四川农业大学动物科技学院, 四川 成都 611130;四川省农业科学院水产研究所, 四川 成都 611731;重庆三峡职业学院, 重庆 404155;四川省长吻鮠原种场, 四川 成都 611247 |
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| 摘 要： | 为了综合评价稻田和池塘两种养殖模式下建鲤(Cyprinus carpio var. jian)的肠道健康及肌肉风味, 在夏秋两季对这两种养殖模式下建鲤的肠道菌群、肠道免疫酶活性及肌肉氨基酸含量进行比较分析。结果显示, 与养殖模式相比较，建鲤肠道菌群受季节的影响更显著。夏秋两季两种模式下建鲤肠道的优势菌群为厚壁菌门(Firmicutes)、梭杆菌门(Fusobacteria)、变形菌门(Proteobacteria), 且池塘模式下秋季肠道菌群多样性显著高于夏季(P<0.05)。建鲤肠道免疫酶活性夏季比秋季显著降低(P<0.05), 其中夏季和秋季池塘的碱性磷酸酶(AKP)活性显著高于稻田模式(P<0.05)。建鲤肌肉中共检测出18种游离氨基酸, 其中肌肉中天冬氨酸、谷氨酸、丙氨酸、甘氨酸、苏氨酸、精氨酸、组氨酸及苯丙氨酸的含量在稻田模式显著高于池塘模式(P<0.05), 且鲜、甜及苦3种呈味氨基酸总体含量均高于池塘模式(P<0.05)。综上所述, 与池塘养殖建鲤相比, 稻田养殖的建鲤肠道菌群更稳定, 肌肉具有更强烈的呈味特性。
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| 关 键 词： | 建鲤 稻田养殖 池塘养殖 肠道菌群 免疫酶活 氨基酸 |
Analysis and comparison of intestinal microbiota, immune enzyme activities, and muscle flavor of Jian carp in two culture modes |
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| Zhao Liulan,Long Yanan,Luo Jie,Liu Qiao,Zhou Jian,Du Jun,Zhou Y,Yang Baiwei,Yang Song.Analysis and comparison of intestinal microbiota, immune enzyme activities, and muscle flavor of Jian carp in two culture modes[J].Journal of Fishery Sciences of China,2021,28(1):48-56. |
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| Authors: | Zhao Liulan Long Yanan Luo Jie Liu Qiao Zhou Jian Du Jun Zhou Y Yang Baiwei Yang Song |
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| Institution: | College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130 , China;Fisheries Institute of Sichuan, Chengdu 611731 , China;Chongqing Three Gorges Vocational College, Chongqing 404155 , China;Sichuan Provincial Governor Kishima Seed Farm, Chengdu 611247 , China |
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| Abstract: | To investigate the differences in the intestinal health and muscle flavor of Jian carp (Cyprinus carpio var. jian) raised in rice field and pond cultivation modes, this experiment compared and analyzed the intestinal microbiota, intestinal immune enzyme activity, and muscle amino acid composition of Jian carp from the two cultivation modes in both summer and autumn. The results showed that the change in intestinal microbiota was related to the seasons. The dominant microbiota under the two modes in summer and autumn were Firmicutes, Fusobacteria, and Proteobacteria, and the diversity of intestinal microbiota in autumn was significantly higher than that in summer under the pond mode (P<0.05). The immune enzyme activity in the intestines was significantly decreased from summer to autumn (P<0.05), and in the summer, the activity of AKP was significantly higher in pond than that in rice field cultivation (P<0.05). A total of 18 types of free amino acids were detected in the muscle of Jian carp. The contents of aspartic acid, glutamic acid, alanine, glycine, threonine, arginine, histidine, and phenylalanine were significantly higher in the rice field (P<0.05), and the total contents of fresh, sweet, and bitter amino acids were all higher in the rice field (P<0.05). In conclusion, under the rice field mode, Jian carp can obtain a more stable intestinal microbiota and a stronger flavor muscle. |
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