| 不同激素组合对人工诱导星康吉鳗性成熟效果的比较 |
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| 引用本文： | 李晓龙,李慷,汝高盟,江兴龙,刘利平.不同激素组合对人工诱导星康吉鳗性成熟效果的比较[J].上海海洋大学学报,2021,30(1):29-38. |
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| 作者姓名： | 李晓龙 李慷 汝高盟 江兴龙 刘利平 |
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| 作者单位： | 水产种质资源发掘与利用教育部重点实验室，上海海洋大学,水产种质资源发掘与利用教育部重点实验室，上海海洋大学,水产种质资源发掘与利用教育部重点实验室,鳗鲡现代产业技术教育部工程研究中心,水产种质资源发掘与利用教育部重点实验室，上海海洋大学 |
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| 基金项目： | 鳗鲡现代产业技术教育部工程研究中心开放基金(RE201803)；中国博士后科学基金面上资助（2018M641984）；中国-东盟海上合作基金项目（DF） |
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| 摘 要： | 通过分析星康吉鳗(Conger myriaster)人工催熟过程中其外表形态、性腺发育和性类固醇激素含量的变化关系,以期为星康吉鳗的人工繁殖提供数据支撑。实验共收集野生星康吉鳗100尾(288.30±62.34)g],分为对照组和3个激素注射组:实验组A(CPE:2 mg/kg),实验组B(HCG:200 IU/kg)和实验组C(CPE:1 mg/kg和HCG:100 IU/kg)。每周进行1次激素注射,共注射12次激素,对照组不做处理。结果表明:实验组C亲鱼卵母细胞发育至细胞核移位阶段,星康吉鳗性腺发育状态最佳。完成12次激素注射后,所有的实验组星康吉鳗卵母细胞发育均达到Ⅲ期,而对照组卵母细胞发育仍停滞在第Ⅱ期。通过对类固醇激素进行ELISA检测表明,在第12次注射后,实验组C星康吉鳗性腺中睾酮含量(217.00±31.76)ng/g]显著高于对照组(123.24±21.05)ng/g]。与对照组相比,注射激素实验组星康吉鳗性腺中17α-羟基孕酮含量呈现缓慢上升趋势。至实验结束时,CPE和HCG组合激素注射的星康吉鳗性腺发育到第Ⅴ期。综上所述,CPE+HCG能够有效地促进星康吉鳗的性腺发育,可作为催熟激素用于星康吉鳗的人工繁殖。
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| 关 键 词： | 星康吉鳗 人工繁殖 性腺发育 类固醇激素 |
| 收稿时间： | 2019/5/14 0:00:00 |
| 修稿时间： | 2020/3/5 0:00:00 |
Comparative analysis of the effects of HCG and CPE combinations on the sexual maturation of common Japanese conger Conger myriaster |
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| LI Xiaolong,LI Kang,RU Gaomeng,JIANG Xinglong,LIU Liping.Comparative analysis of the effects of HCG and CPE combinations on the sexual maturation of common Japanese conger Conger myriaster[J].Journal of Shanghai Ocean University,2021,30(1):29-38. |
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| Authors: | LI Xiaolong LI Kang RU Gaomeng JIANG Xinglong LIU Liping |
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| Institution: | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Educatin, Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Educatin,Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Educatin, Shanghai Ocean University,Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Educatin,Shanghai Ocean University |
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| Abstract: | By analyzing the relationship between gonadal development and sex steroid hormone content during artificial ripening process,the aim of this study is to provide knowledge of artificial induction of maturation in Conger myriaster.A total of 100body mass:(288.30±62.34)g]wild Japanese conger were collected and divided into four groups:group A(CPE:2 mg/kg),group B(HCG:200 IU/kg),group C(CPE:1 mg/kg and HCG:100 IU/kg)and control group without any hormone injection.The injection was given once a week and lasted for 12 weeks.The results showed that hormones can significantly promote the development of oocytes and induced the development of oocytes to the stage of nuclear transplantation successfully.The gonad of fish from Group C had the best development that about 32%eels’oocytes developed intoⅣstage.Moreover,all the fish induced by treatment had developed the oocyte intoⅢstage compared to the control group which was still stagnant in the stageⅡ.The content of testosterone(217.00±31.76)ng/g]in the gonads of conger eels from treatment group C was significantly higher than that from the control group(123.24±21.05)ng/g].This study revealed that the CPE combined with HCG plays a better role in inducing gonad maturation of Conger myriaster. |
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| Keywords: | Conger myriaster artificial reproduction gonadal development steroid hormones |
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