| 抗菌肽不同处理方式对虹鳟免疫指标的影响 |
| |
| 引用本文： | 闫春梅,郑伟,肖志国,韩叶,李忠强,王秀兰.抗菌肽不同处理方式对虹鳟免疫指标的影响[J].上海海洋大学学报,2021,30(1):65-73. |
| |
| 作者姓名： | 闫春梅 郑伟 肖志国 韩叶 李忠强 王秀兰 |
| |
| 作者单位： | 吉林省水产科学研究院,吉林省水产科学研究院,吉林省水产科学研究院,吉林省水产科学研究院,吉林省水产科学研究院,吉林省水产科学研究院 |
| |
| 基金项目： | 吉林省科技发展计划项目(20180201065NY) |
| |
| 摘 要： | 为明确中国鲎基因工程抗菌肽不同处理方式对虹鳟(Oncorhynchus mykiss)存活率的影响,分别在虹鳟仔鱼和成鱼两个阶段采用浸泡和混合饲料两种方式应用抗菌肽。采集仔鱼全鱼组织,成鱼血清及头肾组织,通过对SOD、ALP、CAT、IL-1β、IFN-γ、TNF-α等免疫相关酶水平及细胞因子表达量变化情况的检测,验证抗菌肽不同处理方式对虹鳟免疫指标的影响。结果发现:应用84 U/mL抗菌肽浸泡虹鳟仔鱼10次,浸泡后第4天,试验组免疫相关酶水平明显高于对照组,其中IL-1β、IFN-γ差异极显著(P<0.01),其他差异显著(P<0.05)。浸泡后第15天,试验组免疫相关酶水平仍高于对照组健康鱼,其中IL-1β、IFN-γ差异极显著(P<0.01),其他差异显著(P<0.05),但对照组发病鱼免疫相关酶水平与试验组相比显著下降,SOD、ALP、IL-1β、IFN-γ均差异极显著(P<0.01),其他差异显著(P<0.05)。试验结束统计仔鱼存活率,试验组存活率高达95.1%,对照组存活率仅为29.9%。成鱼期采用混合饲料和浸泡两种方式应用抗菌肽。结果显示,混合饲料的最适添加量为50 mg/kg饲料,浸泡最佳浓度为84 U/mL,最佳浸泡时间为3 min。研究表明,对不同处理方式进行对比,在仔鱼期进行浸泡对虹鳟免疫指标的影响最为显著。其可能与抗菌肽种类、作用方式及养殖虹鳟疾病暴发特点有关。本研究可以为虹鳟养殖过程中抗菌肽的应用方式提供依据。
|
| 关 键 词： | 虹鳟 抗菌肽 处理方式 免疫相关酶 细胞因子 |
| 收稿时间： | 2020/2/27 0:00:00 |
| 修稿时间： | 2020/4/27 0:00:00 |
Effects of different treatments of antimicrobial peptides on immune indexes of rainbow trout(Oncorhynchus mykiss) |
| |
| YAN Chunmei,ZHENG Wei,XIAO Zhiguo,HAN Ye,LI Zhongqiang,WANG Xiulan.Effects of different treatments of antimicrobial peptides on immune indexes of rainbow trout(Oncorhynchus mykiss)[J].Journal of Shanghai Ocean University,2021,30(1):65-73. |
| |
| Authors: | YAN Chunmei ZHENG Wei XIAO Zhiguo HAN Ye LI Zhongqiang WANG Xiulan |
| |
| Institution: | Jilin Fisheries Research Institute,Changchun,130033;P.R.China,Jilin Fisheries Research Institute,Changchun,130033;P.R.China,Jilin Fisheries Research Institute,Changchun,130033;P.R.China,Jilin Fisheries Research Institute,Changchun,130033;P.R.China,Jilin Fisheries Research Institute,Changchun,130033;P.R.China,Jilin Fisheries Research Institute,Changchun,130033;P.R.China |
| |
| Abstract: | To clarify the effects of different treatments of Chinese horseshoe crab genetic engineering antimicrobial peptide on the survival rate of rainbow trout,in this study,the antimicrobial peptides were applied by soaking and mixed feed in the larvae and adult stages of rainbow trout respectively.In order to verify the effects of different treatments of antimicrobial peptides on immune indexes of rainbow trout,the whole fish tissues of the larvae and the serum and head kidney tissues of the adult fish were collected respectively to detect the levels of the immune-related enzymes of SOD,ALP,CAT,IL-1β,IFN-γ,and TNF-α.The results showed that:soaking 10 times with 84 U/mL antimicrobial peptides of the larvae of rainbow,the immune-related enzyme levels of the experimental group were significantly higher than those in the control group on the 4 th day after soaking,such as IL-1βand IFN-γwere extremely significant(P<0.01),while the others were significant(P<0.05);on the 15 th day after soaking,the experimental group was still higher than the healthy fish of control group,IL-1βand IFN-γwere extremely significant(P<0.01),while the others were significant(P<0.05).However,the levels of immune-related enzymes in the sick fish of control group were decreased significantly than those of the experimental group.SOD,ALP,IL-1β,IFN-γwere extremely significant(P<0.01),while the others were significant(P<0.05).The survival rate of larvae in the experimental group was up to 95.1%,while the control group was only 29.9%.The adult fish were carried out from two aspects:mixed feed test and soaking test.The results showed that,the optimal dosage of the mixed feed was 50 mg/kg,the optimal concentration of the soaking was 84 U/mL,and the optimal soaking time was 3 min.Compared with the different treatments,it is found that the effects of soaking on the immune index of rainbow trout were most significant at the stage of the larvae.It may be related to the type of antimicrobial peptides,the mode of action and the outbreak characteristics of farmed rainbow trout.The results of this study will provide experimental basis for the application of antimicrobial peptides in rainbow trout culture. |
| |
| Keywords: | antimicrobial peptides treatments immune-related enzymes cytokines |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《上海海洋大学学报》浏览原始摘要信息 |
| 点击此处可从《上海海洋大学学报》下载免费的PDF全文 |
|
