| 功能基因组注释探讨解淀粉芽孢杆菌的益生机制 |
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| 引用本文： | 张红娜,周玉法,庞全海.功能基因组注释探讨解淀粉芽孢杆菌的益生机制[J].西北农业学报,2020,30(12):1883-1890. |
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| 作者姓名： | 张红娜 周玉法 庞全海 |
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| 作者单位： | （1 .河北经贸大学 生物科学与工程学院，石家庄 050061；2.山西农业大学 动物科学学院，山西太谷 030801；3.泰安市岱岳区畜牧局，山东泰安 271000） |
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| 基金项目： | 国家自然科学基金（81903282）。 |
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| 摘 要： | 旨在利用功能基因组注释的方法，深入挖掘解淀粉芽孢杆菌BLCC1-0238基因组中与益生作用密切相关的功能基因。结果表明，解淀粉芽孢杆菌BLCC1-0238的基因组大小约为3.88 Mb，含有3 941个编码序列，有8个基因簇参与聚酮类化物、非核糖体脂肽和芽孢杆菌素的生物合成，部分基因簇参与环状脂肽罗克霉素的合成；该菌还具有1个编码肽基脯氨酰异构酶的基因，12个与耐酸相关的基因和7个参与编码水解酶的基因。综上所述，这些基因的存在有助于动物机体对抗病原菌和胁迫因素，还有利于对营养物质的消化和 吸收。
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| 关 键 词： | 解淀粉芽孢杆菌 功能基因组 次级代谢产物 益生机制 基因簇 |
Annotation and Discussion on Beneficial Properties of Probiotic Bacillus amyloliquefaciens by Use of Functional Genome |
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| ZHANG Hongn,ZHOU Yufa and PANG Quanhai.Annotation and Discussion on Beneficial Properties of Probiotic Bacillus amyloliquefaciens by Use of Functional Genome[J].Acta Agriculturae Boreali-occidentalis Sinica,2020,30(12):1883-1890. |
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| Authors: | ZHANG Hongn ZHOU Yufa and PANG Quanhai |
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| Abstract: | The purpose of this study is to dig deep into functional genes closely related to beneficial properties in the genome of Bacillus amyloliquefaciens BLCC1-0238 by functional genome annotation. The results showed that the genome size of B.amyloliquefaciens was approximate 3.88 Mb with 3 941 encoding sequences, 8 gene clusters were involved in the synthesis of non-ribosomal lipopeptides, polyketides, and bacilysin, and some gene clusters participated in the synthesis of cyclic lipopeptide.In addition,1 gene encoding a peptidylprolyl isomerase,12 genes related with acid tolerance,and 7 genes encoding hydrolase were found in the genome of B.amyloliquefaciens BLCC1-0238. In conclusion, these genes help animal hosts combat pathogens and stress factors, and improve the digestion and absorption of nutrition. |
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| Keywords: | Bacillus amyloliquefaciens Functional genome Secondary metabolites Beneficial property Gene cluster |
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