| 条锈菌诱导的小麦C3HC4型锌指蛋白基因的克隆及其特征分析 |
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| 引用本文： | 张 毅,张 岗,徐 进,郭 军,黄丽丽,康振生.条锈菌诱导的小麦C3HC4型锌指蛋白基因的克隆及其特征分析[J].西北农业学报,2010,19(10):49-54. |
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| 作者姓名： | 张 毅 张 岗 徐 进 郭 军 黄丽丽 康振生 |
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| 作者单位： | 1. 西安市植保植检站,西安,710061
2. 西北农林科技大学,植物保护学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目(2006CB708208); 国家高新技术研究发展计划(863计划)重大研究项目(2006AA10A104); 教育部科学技术研究重点项目(107104); 教育部长江学者和创新团队发展计划项目(IRT0558); 国家自然科学基金项目(30671350); 高等学校学科创新引智计划项目(B07049) |
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| 摘 要： | 应用电子克隆和RT-PCR方法,从小麦叶片中首次分离出1个条锈菌诱导的编码C3HC4型锌指蛋白基因的cDNA序列,暂被命名为TaZFP1。TaZFP1包含一个完整的849 bp的开放阅读框,编码282个氨基酸,具有C3HC4保守结构域;小麦TaZFP1氨基酸序列与水稻OsZFP1相似性达89%,与玉米ZmZFP1相似性达81%。TaZFP1在小麦根、茎、叶组织中表达量基本一致。在小麦与条锈菌的亲和、非亲和互作中,TaZFP1基因均受条锈菌诱导高水平表达,且非亲和组合表达量高于亲和组合,表明TaZFP1可能参与小麦对条锈菌的防御反应。
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| 关 键 词： | 条锈菌 小麦 C3HC4型锌指蛋白 电子克隆 基因表达 |
Cloning and Characterization of a C3HC4 type Zinc Finger Gene in Wheat Induced by Stripe Rust Pathogen |
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| ZHANG Yi,ZHANG Gang,XU Jin,GUO Jun,HUANG Lili and KANG Zhensheng.Cloning and Characterization of a C3HC4 type Zinc Finger Gene in Wheat Induced by Stripe Rust Pathogen[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(10):49-54. |
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| Authors: | ZHANG Yi ZHANG Gang XU Jin GUO Jun HUANG Lili and KANG Zhensheng |
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| Institution: | ZHANG Yi1,ZHANG Gang2,XU Jin1,GUO Jun2,HUANG Lili2 and KANG Zhensheng2 (1.Xi'an Plant Protection and Quarantine Station,Xi'an 710061,China,2.College of Plant Protection and Shaanxi Provincial Key Laboratory of Molecular Biology for Agriculture,Northwest A&F University,Yangling Shaanxi 712100,China) |
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| Abstract: | To better understand wheat(Triticum aestivum L.) defense responses to Puccinia striiformis f.sp.tritici,the cDNA library of wheat leaves infected by Puccinia striiformis f.sp.tritici is constructed in our laboratory.On the basis of previous study,a new C3HC4-type zinc finger gene was isolated from this cDNA library through in-silico cloning and RT-PCR approaches.The gene was tentatively designated as TaZFP1,whose open reading frame was 849 bp in length and encoded 282 amino acids containing a conserved C3HC... |
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| Keywords: | Stripe rust fungus Wheat C3HC4 type zinc finger In-silico cloning Gene expression |
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