| 鸡新城疫病毒洛阳分离株HN基因的克隆和序列分析 |
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| 引用本文： | 余祖华,程相朝,张春杰,李银聚,吴庭才,丁轲,何雷.鸡新城疫病毒洛阳分离株HN基因的克隆和序列分析[J].河南农业科学,2006(12):82-86. |
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| 作者姓名： | 余祖华 程相朝 张春杰 李银聚 吴庭才 丁轲 何雷 |
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| 作者单位： | 河南科技大学动物科技学院,河南,洛阳,471003 |
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| 摘 要： | 应用RT PCR技术从新城疫病毒洛阳分离株中扩增HN的cDNA片段,并将其克隆至pMD18 T载体进行核苷酸序列测定。结果表明:新城疫分离株HN基因片段长度为1716bp,编码571个氨基酸;推测出的氨基酸序列中有5个糖基化位点,12个半胱氨酸残基;与国内外的14株新城疫毒株HN基因相比,核苷酸和氨基酸同源性分别为66.4%~98.5%和87.8%~98.3%;与标准毒株Lasota和Clone30的亲缘关系较远,核苷酸和氨基酸差异性较大,而与天津分离株和云南分离株的亲缘关系较近,基本属于同一基因群。
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| 关 键 词： | 新城疫病毒 HN基因 序列分析 同源性比较 |
| 文章编号： | 1004-3268(2006)12-0082-05 |
| 收稿时间： | 2006-08-16 |
| 修稿时间： | 2006年8月16日 |
Cloning and Sequencing of HN Gene of Newcastle Disease Virus Isolated from Luoyang |
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| YU Zu-hua,CHENG Xiang-chao,ZHANG Chun-jie,LI Yin-ju,WU Ting-cai,DING Ke,HE Lei.Cloning and Sequencing of HN Gene of Newcastle Disease Virus Isolated from Luoyang[J].Journal of Henan Agricultural Sciences,2006(12):82-86. |
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| Authors: | YU Zu-hua CHENG Xiang-chao ZHANG Chun-jie LI Yin-ju WU Ting-cai DING Ke HE Lei |
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| Institution: | College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China |
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| Abstract: | The cDNA fragments of HN gene of Newcastle disease virus(NDV) from Luoyang were amplified by RTPCR and cloned into pMD18T vector,the recombinant plasmids were sequenced.The results showed that the HN gene fragments consisted of 1716bp coding for 571(amino) acids which contained 5 glycosylation sites and 12 cysteine residues.Compared with the other 14 Newcastle disease virus strains discovered,the sequence homology of neucletide and amino acids were from 66.4% to 98.5% and from 87.8% to 98.3%,respectively.The virus insolated from Luoyang had a further distance with the standard virus lasota and cloning 30,and was closer with the virus strains from Tianjin and Yunnan. |
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| Keywords: | Newcastle disease virus HN gene Sequence analysis Homology |
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