| 苎麻茎尖的组织培养及其诱导植株的再生 |
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| 引用本文： | 郭运玲,郭安平,刘恩平,孔华,贺立卡.苎麻茎尖的组织培养及其诱导植株的再生[J].热带作物学报,2006,27(1):73-76. |
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| 作者姓名： | 郭运玲 郭安平 刘恩平 孔华 贺立卡 |
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| 作者单位： | 中国热带农业科学院热带作物生物技术国家重点实验室,海口,571101;中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 摘 要： | 对苎麻50号品系的茎尖进行组织培养并诱导植株再生。结果表明:最适茎尖转绿和分化的培养基是1/2MS 6-BA1.0mg/L CH300mg/L的液体培养基,茎尖分化率为100%。最适成苗培养基为MS 6-BA0.5mg/L IAA0.1mg/L CH300mg/L,成苗率是53.3%。扩繁培养基为MS 6-BA0.3￣1.0mg/L,繁殖系数达6.7。适宜生根培养基为1/2MS NAA0.01mg/L。最佳茎尖剥取材料是无菌试管苗,茎尖长以0.3mm带1￣2个叶原基为宜。从茎尖接种培养成苗到移栽需4个月左右。
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| 关 键 词： | 苎麻 茎尖 培养基 植株再生 |
| 收稿时间： | 2005-05-11 |
| 修稿时间： | 2005年5月11日 |
Shoot Tip Culture and Plantlet Regeneration in Ramie |
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| Guo Yunling,Guo Anping,Liu Enping,Kong Hua,He Lika.Shoot Tip Culture and Plantlet Regeneration in Ramie[J].Chinese Journal of Tropical Crops,2006,27(1):73-76. |
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| Authors: | Guo Yunling Guo Anping Liu Enping Kong Hua He Lika |
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| Institution: | State Key Laboratory of Tropical Crop Biotechnology/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China |
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| Abstract: | Line 50 of ramie Boehmeria nivea (L.)Gaud] was used as a sample to study its shoot-tip culture and plantlet regeneration. The results showed that the liquid medium MS 6-BA 1.0 mg/L CH 300 mg/L was best for greening and differentiation of explants in vitro with a 0.3 mm long shoot tip having 1-2 leaf primordia. The best medium for plantlet regeneration was MS 6-BA 0. 5 mg/L IAA 0.1 mg/L CH 300 mg/L with an efficiency of 53.3 %. For rapid propagation the medium used was MS 6-BA0.3-1.0mg/L with a multiplication rate of 6.7. The suitable rooting medium was 1/2 MS NAA 0.01 mg/L. It took about 4 months for the shoot tip to develop into rooted plantlets. |
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| Keywords: | ramie shoot tips medium plantlet regeneration |
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