| 提取东北虎毛发DNA两种方法的比较研究 |
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| 引用本文： | 吴云良,陈晶,陈清,张红霞,陈国宏.提取东北虎毛发DNA两种方法的比较研究[J].中国畜牧兽医,2007,34(9):33-35. |
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| 作者姓名： | 吴云良 陈晶 陈清 张红霞 陈国宏 |
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| 作者单位： | 扬州大学动物科学与技术学院，扬州 225009 |
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| 基金项目： | 江苏省六大人才高峰基金 |
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| 摘 要： | 研究东北虎毛发DNA的水煮抽提法，并将传统的酚仿抽提法和水煮法进行比较。水煮法即通过对细胞的煮沸和冷却，使细胞破裂、蛋白质变性，从而获得用于PCR扩增的模板DNA。通过测定OD值并计算浓度和纯度，同时进行琼脂糖电泳检测、PCR扩增和PAGE电泳检测，分析2种方法提取东北虎毛发DNA的质量差异。结果表明：水煮法提取东北虎毛发中DNA是一种快捷、简便、经济、高效的方法。
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| 关 键 词： | 东北虎 毛发 DNA 酚仿抽提法 水煮抽提法 |
| 文章编号： | 1671-7236（2007）09-0033-03 |
| 修稿时间： | 2007-03-06 |
Comparative Study of Two DNA Extraction Methods from the Hairs of the Panthera Tigris Altaica |
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| WU Yun-liang,CHEN Jing,CHEN Qing,ZHANG Hong-xia,CHEN Guo-hong.Comparative Study of Two DNA Extraction Methods from the Hairs of the Panthera Tigris Altaica[J].China Animal Husbandry & Veterinary Medicine,2007,34(9):33-35. |
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| Authors: | WU Yun-liang CHEN Jing CHEN Qing ZHANG Hong-xia CHEN Guo-hong |
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| Institution: | College of Animal Science and Technology in Yangzhou University, Yangzhou 225009, China |
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| Abstract: | The DNA extraction from the hairs of the Panthera Tigris Altaica,cooking extraction method in water was studied,and the comparison with the traditional phenol and chloroform method was done.The cooking extraction method was the method from which genomic DNA was obtained when the cells were broken and proteins were denatured after the cells were cooked and cooled quickly.The extracting results of the two methods were detected through agrose gel electrophoresis,PCR amplification and PAGE gel electrophoresis,the OD value,density and purity.The analysis result indicated that the cooking extraction method in water is a quick,simple,economic and efficient method to extract DNA from the hairs of the Panthera Tigris Altaica. |
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| Keywords: | DNA |
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