| 合欢叶提取物对油菜菌核病菌的抑制作用及其苗期防治效果 |
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| 引用本文： | 刘峰,代光辉,周熙荣,陈佳,李会.合欢叶提取物对油菜菌核病菌的抑制作用及其苗期防治效果[J].植物病理学报,2010,40(5):511-516. |
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| 作者姓名： | 刘峰 代光辉 周熙荣 陈佳 李会 |
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| 作者单位： | 1 上海交通大学农业与生物学院农业部都市农业(南方)开放重点实验室, 上海 200240;2 上海市农业科学院作物育种栽培研究所, 上海 201106 |
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| 基金项目： | 国家科技部农业科技成果转化基金，上海市蔬菜学重点学科基金 |
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| 摘 要： | 不同浓度的合欢叶提取物对油菜菌核病菌(Sclerotinia sclerotiorum)菌丝生长及菌核形成的抑制试验表明,该提取物具有抑制菌丝生长及菌核形成的作用,且随着浓度的提高活性增强,当浓度为20 mg/mL时,对菌丝生长的抑制率为73.1%,对菌核形成的抑制率为100%。通过对提取物处理后菌丝形态显微观察,发现病菌菌丝出现形态异常甚至断裂现象,且培养液电导率增加,推测为提取物处理后菌丝细胞壁破裂、细胞质外渗造成。苗期盆栽试验发现,该提取物对油菜菌核病菌有明显的防治效果,当浓度为20 mg/mL时,防治效果达72.1%。
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| 关 键 词： | 合欢 植物提取物 油菜菌核病菌 抑制作用 防治效果 |
Inhibition of extracts from the leaves of Albizzia julibrissin to Sclerotinia sclerotiorum and control effect at the seedling stage |
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| LIU Feng,DAI Guang-hui,ZHOU Xi-rong,CHEN Jia,LI Hui.Inhibition of extracts from the leaves of Albizzia julibrissin to Sclerotinia sclerotiorum and control effect at the seedling stage[J].Acta Phytopathologica Sinica,2010,40(5):511-516. |
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| Authors: | LIU Feng DAI Guang-hui ZHOU Xi-rong CHEN Jia LI Hui |
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| Institution: | 1 Key Laboratory of Urban Agriculture(South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China;2 Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China |
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| Abstract: | The study showed that the crude extracts from leaves of Albizzia julibrissin Durazz had inhibition effect against the mycelium growth and sclerotial formation of Sclerotinia sclerotiorum in a dose-dependent manner, with the inhibition rate 73.1% for mycelia growth and 100% for sclerotial formation at concentration of 20 mg/mL. The microscope observation showed that the hyphae of S. sclerotiorum were deformed after being treated with the plant extracts, leading to a cytoplasm exudation and the increase of electrical conductivity of the mycelium suspension of the pathogen. The control effect (72.1%) of the extracts (20 mg/mL) against the disease was demonstrated at the seedling stage through pot culture experiment. |
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| Keywords: | Albizzia julibrissin Durazz extracts Sclerotinia sclerotiorum inhibition control effect |
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