| 巴西蘑菇多糖诱导K562肿瘤细胞凋亡的研究 |
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| 引用本文： | 袁丽铭,忻茜,李兴玉.巴西蘑菇多糖诱导K562肿瘤细胞凋亡的研究[J].食用菌学报,2005,12(3):38-42. |
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| 作者姓名： | 袁丽铭 忻茜 李兴玉 |
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| 作者单位： | 上海师范大学生命与环境科学学院,上海,200234 |
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| 基金项目： | 上海市高等院校科学技术发展基金项目“巴西蘑菇DNA反义核酸抗肿瘤的实验研究”（编号：02DK09）的部分研究内容 |
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| 摘 要： | 应用流式细胞分选仪（Flow cell sorter,FCS）和透射电子显微镜（Transmissional electron microscope,TEM）等技术观察了巴西蘑菇（Agaricus blazei）多糖诱导K562肿瘤细胞凋亡的试验研究.试验发现,流式细胞仪可检测到经巴西蘑菇多糖诱导K562肿瘤96h后产生明显的亚二倍体峰;电镜下观察到K562肿瘤细胞核固缩,核内染色质浓缩、凝聚,紧靠在细胞核膜边沿,形成新月状或环状结构等典型的凋亡细胞的形态变化及凋亡小体.结果表明,巴西蘑菇多糖具有诱导K562肿瘤细胞凋亡的作用.
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| 关 键 词： | 巴西蘑菇多糖 诱导 K562肿瘤细胞 细胞凋亡 |
| 文章编号： | 1005-9873（2005）03-0038-05 |
| 收稿时间： | 2005-02-23 |
| 修稿时间： | 2005-04-25 |
The Apoptosis of K562 Tumor Cells Induced by Polysaccharide Extracted from Agaricus blazei |
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| YUAN Li-ming,XIN Xi,LI Xing-yu.The Apoptosis of K562 Tumor Cells Induced by Polysaccharide Extracted from Agaricus blazei[J].Acta Edulis Fungi,2005,12(3):38-42. |
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| Authors: | YUAN Li-ming XIN Xi LI Xing-yu |
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| Institution: | Life and Science College, Shanghai Normal University, Shanghai 200234, China |
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| Abstract: | The research on the apoptosis of K562 tumor cells induced by polysaccharide extracted from Agaricus blazei was carried out using advanced technology of flow cell sorter and transmissional electron microscope. The result showed that, visible apoptotic peak could be detected by flow cell sorter after being induced for 96h by polysaccharide extracted from Agaricus blazei. Furthermore, karyopyknosis, chromatin condensed and aggregated, hugging the edge of cell nucleus, showing crico-structure, crescent-structure and other typical morphologic changes of apoptotic cell as well as apoptotic body could be seen in K562 cells through electro-microscope. It was concluded that the apoptosis of K562 tumor cells could be induced by polysaccharide extracted from Agaricus blazei . |
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| Keywords: | Polysaccharide extracted from Agaricus blazei Induce K562 tumor cell Apoptosis |
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