The Complete Nucleotide Sequence of a Japanese Isolate of White clover mosaic virus Strain RC |
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| Authors: | Hitomi NAKABAYASHI Yasuyuki YAMAJI Satoshi KAGIWADA Masashi UGAKI Shigetou NAMBA |
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| Institution: | (1) Laboratory of Bioresource Technology, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 202 Bioscience Bldg., Kashiwa 277-8562, Japan, JP |
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| Abstract: | The complete nucleotide sequence was determined for genomic RNA of White clover mosaic virus (WClMV-RC) isolated from red clover (Trifolium pratense) in Japan, It is 5843 nucleotides in length, excluding the poly(A) tail at the 3' terminus. Similar to other potexviruses,
it contains five open reading frames (ORFs 1 through 5), which putatively encode an RNA-dependent RNA polymerase (RdRp) (147
kDa), a triple gene block (TGB) (26 kDa/13 kDa/7 kDa), and a coat protein (CP) (22 kDa), respectively. The deduced amino acid
sequence of the WClMV-RC CP was identical to that of WClMV-O, one of two New Zealand isolates, but only 85% identical to that
of WClMV-M, the other New Zealand isolate, because of heterogeneity in the C-termini of CP amino acid sequences. The implication
of this CP heterogeneity is discussed.
Received 30 August 2001/ Accepted in revised form 11 January 2002 |
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| Keywords: | : White clover mosaic virus RC strain nucleotide sequence phylogenetic analysis Potexvirus |
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