| 红螯螯虾胚胎发育过程中的生化成分变化 |
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| 引用本文： | 罗文,赵云龙,曾错,崔丽丽,李嘉尧,杨受保.红螯螯虾胚胎发育过程中的生化成分变化[J].水产学报,2007,31(3). |
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| 作者姓名： | 罗文 赵云龙 曾错 崔丽丽 李嘉尧 杨受保 |
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| 基金项目： | 上海市科技兴农科技攻关项目 |
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| 摘 要： | 采用生物化学方法对红螯螯虾胚胎发育过程中主要生化成分(糖类、蛋白质及氨基酸、脂类及脂肪酸)进行了测定和分析,以期了解胚胎形态发生与营养需求的关系.结果显示:红螯螯虾胚胎发育过程中糖类总体含量很低.总蛋白与总氨基酸(TAA)的含量在胚胎发育过程中均呈下降趋势.在必需氨基酸(EAA)中亮氨酸(Leu)和精氨酸(Arg)的含量较高,而在非必需氨基酸中谷氨酸(Glu)和天冬氨酸(Asp)的含量较高.总脂在胚胎发育过程中的含量总体呈下降趋势,其中性脂和磷脂的主要脂肪酸均为C16:0、C18:0 、C18:1ω9和C18:3ω3.研究认为:在红螯螯虾胚胎发育过程中,糖类主要参与多种特异复合物的合成以及甲壳构建,这些特异复合物在细胞分化中起着重要的信号传递作用.蛋白质和脂类都是红螯螯虾胚胎发育过程中主要的结构和能源物质,蛋白质主要在早期被利用而脂类主要在晚期起作用.饱和脂肪酸(SFA)和单不饱和脂肪酸(MUFA)(C16:0,C18:0,C16:1ω7 和 C18:1ω9)主要作为能源物质被消耗而高不饱和脂肪酸(PUFA)(20:5ω3和22:6ω3)在胚胎发育过程中对于构建细胞膜以及调节中枢神经系统的发育起重要作用.
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| 关 键 词： | 红螯螯虾 胚胎发育 蛋白质 氨基酸 脂肪 脂肪酸 |
Biochemical changes during the embryonic development of redclaw crayfish, Cherax quadricarinatus |
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| LUO Wen,ZHAO Yun-long,ZENG Cuo,CUI Li-li,LI Jia-yao,YANG Shou-bao.Biochemical changes during the embryonic development of redclaw crayfish, Cherax quadricarinatus[J].Journal of Fisheries of China,2007,31(3). |
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| Authors: | LUO Wen ZHAO Yun-long ZENG Cuo CUI Li-li LI Jia-yao YANG Shou-bao |
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| Abstract: | The objective of the present study was to investigate the biochemical changes during embryogenesis of Cherax quadricarinatus, in order to evaluate the nutritional requirements of embryos. The carbohydrate content remained a minor constituent of yolk on the whole. The total protein and the total amino acids (TAA) were all observed down-trend during embryonic development. In the essential amino acids (EAA), the content of leucine and arginine acid were relatively high, but in the non-essential amino acid (NEAA), the quantitatively most important were glutamine and aspartic acid. The content of total lipid decreased during embryonic development, and the predominant fatty acids of both neutral lipid and polar lipid were all C16:0, C18:0, C18:1ω9 and C18:3ω3. It's indicated that the carbohydrate played an important role in synthesizing many specific compounds to participate in signal transmission and to form carapace. The protein and lipid were all the dominating construction and energy substances; protein was primarily consumed in the early stages but lipid in the late stages. The saturated fatty acids (SFA) and the monounsaturated fatty acids (MUFA) (C16:0, C18:0, C16:1ω7 and C18:1ω9) were always used for energetic purpose and the polyunsaturated fatty acids (PUFA) (C20:5ω3 and C22:6ω3) were important as structural components of cell membranes and the central nervous system during the development. |
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| Keywords: | Cherax quadricarinatus embryogenesis protein amino acid lipid fatty acid |
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