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| 鸡传染性喉气管炎病毒gD基因在重组痘病毒中表达及其免疫效力 | |
| 引用本文： | 杨明凡,崔保安,陈红英,张素梅,魏战勇,李祥瑞.鸡传染性喉气管炎病毒gD基因在重组痘病毒中表达及其免疫效力[J].南京农业大学学报,2009,32(1). |
| 作者姓名： | 杨明凡 崔保安 陈红英 张素梅 魏战勇 李祥瑞 |
| 作者单位： | 1. 南京农业大学动物医学院,江苏南京210095;河南农业大学牧医工程学院,河南郑州450002 2. 河南农业大学牧医工程学院,河南郑州,450002 3. 南京农业大学动物医学院,江苏南京210095: |
| 摘 要： | 将鸡传染性喉气管炎病毒(ILTV) 河南长葛株gD基因重组到鸡痘病毒基因组中,获得了能高效表达糖蛋白D的重组鸡痘病毒(rFPV-gD) .将rFPV-gD经皮下刺种途径接种30日龄的固始鸡,用ILTV商品弱毒疫苗经滴鼻、点眼途径免疫试验鸡,同时设非免疫对照鸡群.分别于免疫后7、14、21、28、35和42 d采血分离血清,用微量血清中和试验测定ILTV抗体效价,并于免疫后42 d用ILTV强毒攻击所有试验鸡只.血清中和抗体检测结果表明:疫苗对照组与重组病毒组差异不显著(P>0.05) ,而重绀病毒组和疫苗对照组与空白对照组相比筹异均显著(P<0.05) .免疫42 d攻毒后鸡的发病情况表明,重组病毒组和疫苗对照组对ILTV强毒攻击的保护率均为96.67%,而空白对照组为6.67%,说明该重组病毒作为弱毒疫苗能够抵抗ILTV强毒的攻击,对免疫鸡群有较好的保护作用. |
| 关 键 词： | 传染性喉气管炎病毒 gD基因 重组病毒 免疫效力 |
Expression of glycoprotein D(gD) of infectious laryngotracheitis virus in recombinant fowl pox virus and its immune efficacy |
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| YANG Ming-fan,CUI Bao-an,CHEN Hong-ying,ZHANG Su-mei,WEI Zhan-yong,LI Xiang-rui.Expression of glycoprotein D(gD) of infectious laryngotracheitis virus in recombinant fowl pox virus and its immune efficacy[J].Journal of Nanjing Agricultural University,2009,32(1). | |
| Authors: | YANG Ming-fan CUI Bao-an CHEN Hong-ying ZHANG Su-mei WEI Zhan-yong LI Xiang-rui |
| Institution: | 1.College of Veterinary Medicine;Nanjing Agricultural University;Nanjing 210095;China;2.College of Animal Medicine;Henan Agricultural University;Zhengzhou 450002;China |
| Abstract: | A recombinant fowl pox expressing glycoprotein D(gD)of infectious laryngotracheitis virus(ILTV)was constructed by fusing the gD gene of the virus isolated from Changge,Henan,into the genome of fowl pox virus and was named as rFPV-gD.The recombinant virus was then subcutaneously inoculated into 30-old-day chickens.Another group of animals were vaccinated with a commercial ILTV vaccine(D-805)as positive control.The negative control group was not immunized.The ILTV antibody titers were checked by micro serum n... |
| Keywords: | infectious laryngotracheitis virus glycoprotein D gene recombinant virus immune effects |
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