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| 高寒地区红豆草根瘤菌抗逆性研究 | |
| 引用本文： | 朱瑞芬,张榕,师尚礼,高占琪,陈积山.高寒地区红豆草根瘤菌抗逆性研究[J].甘肃农业大学学报,2009,44(4). |
| 作者姓名： | 朱瑞芬 张榕 师尚礼 高占琪 陈积山 |
| 作者单位： | 1. 甘肃农业大学草业学院,甘肃,兰州730070;草业生态系统教育部重点实验室,中–美草地畜牧业可持续发展研究中心,甘肃,兰州,730070 2. 甘肃省草原总站,甘肃,兰州730070 3. 黑龙江省农业科学院草业研究所,黑龙江,哈尔滨150086 |
| 基金项目： | 国家科技部项目，农业部行业专项 |
| 摘 要： | 从甘南合作、甘南夏河桑科的红豆草(Onobrychis vicilfolia)根系中分离培养得到28个根瘤菌菌株,针对各菌株的耐盐性、耐酸碱及其泌酸碱能力进行研究,结果表明:采集于桑科的红豆草根瘤编号STD111ⅠA、STD26ⅠB、STD411ⅠB、STF11ⅡA的菌株耐盐性极强,在10%NaCl培养基上正常生长;采集于合作的红豆草根瘤菌株HND26ⅠA、HND26ⅠB、HND26Ⅱ和桑科红豆草根瘤菌株STD111ⅠA、STD26ⅠB、STD411ⅠA、STD411ⅡA耐酸性最强,能在pH4.0的培养基上正常生长;耐碱性最强的是夏河地区菌株STF11ⅢA,可以在pH12.0的培养基上正常生长.进一步试验表明,菌株HND26ⅠA、HND26ⅠB、HND26Ⅱ、STD111ⅠA和STD26ⅠB既能抗pH 4.0的酸逆境,又能抗pH11.0的碱逆境.同时研究发现,菌株耐碱性的强弱与其泌酸能力相关. |
| 关 键 词： | 红豆草 根瘤菌 耐盐性 耐酸碱性 泌酸碱能力 |
Research on stress resistence of sainfoin rhizobium in alpine region |
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| ZHU Rui-fen,ZHANG Rong,SHI Shang-li,GAO Zhan-qi,CHEN Ji-shan.Research on stress resistence of sainfoin rhizobium in alpine region[J].Journal of Gansu Agricultural University,2009,44(4). | |
| Authors: | ZHU Rui-fen ZHANG Rong SHI Shang-li GAO Zhan-qi CHEN Ji-shan |
| Institution: | ZHU Rui-fen1,2,ZHANG Rong3,SHI Shang-li3,GAO Zhan-qi3,CHEN Ji-shan4(1.College of Prataculture,Gansu Agricultural University,Lanzhou 730070,China,2.Key Laboratory of Grassland Ecosystem Ministry of Education,Sino-U S Center for Grazingland Ecosystem Sustainability,3.Gansu Grassland Working Station,4.Institute of Pratacultural Science of Heilongjiang Agricultural Institute,Herbin 150086,China) |
| Abstract: | 28 rhizobia strains were isolated from sainfoin roots from Hezuo and Sangke in Gansu Province,the salt-resistance,acid-alkali resistance and acid-alkali secreting ability of each strains were studied.The results showed that sainfoin rhizobia strains numbered as STD111ⅠA,STD26ⅠB,STD411ⅠB,STF11ⅡA which collected from Sangke area had the strongest salt-resistant ability,and all of them could regularly grow in 10 % NaCl medium;sainfoin rhizobia strains numbered as HND26ⅠA,HND26ⅠB,HND26Ⅱ which collected from Hezuo area and STD111ⅠA,STD26ⅠB,STD411ⅠA,STD411ⅡA from Sangke area had the strongest acid-resistant ability,and these strains could regularly grow in medium with pH value of 4.0;the strain numbered as STF11ⅢA from Xiahe area owns the strongest alkali-resistant ability,and it could regularly grow in medium with pH value of 12.0.A further experiment indicated that strains numbered as HND26ⅠA,HND26ⅠB,HND26Ⅱ,STD111ⅠA and STD26ⅠB could resist both acid stress with pH value of 4.0 and alkali stress with pH value of 11.0.Meanwhile,the ability of alkali resistence was correlated to the ability of acid secreting. |
| Keywords: | sainfoin rhizobia saline tolerance acid and alkali tolerance acid-alkali secreting ability |
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