| 利用基因芯片筛选甘蔗成熟期叶片差异表达候选基因 |
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| 引用本文： | 黄峰,何文锦,代容春,林荣华,叶冰莹,陈由强.利用基因芯片筛选甘蔗成熟期叶片差异表达候选基因[J].亚热带农业研究,2012,8(1):46-50. |
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| 作者姓名： | 黄峰 何文锦 代容春 林荣华 叶冰莹 陈由强 |
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| 作者单位： | 1. 福建师范大学生命科学学院,福建福州350108／农业部福建甘蔗生物学与遗传育种重点实验室,福建福州350002
2. 福建师范大学生命科学学院,福建福州,350108 |
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| 基金项目： | 农业部现代农业产业技术体系建设专项资金，国家自然科学基金资助项目 |
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| 摘 要： | 利用Agilent甘蔗4×44k(per array)芯片分析甘蔗叶片在甘蔗成熟期的差异表达基因,并通过生物信息学手段对基因芯片杂交结果进行分析。芯片杂交结果符合质控标准,并且杂交数据重复性良好,结果可靠。在甘蔗生长过程中8个时间点上,共筛选出相关差异在2倍以上的基因20个,其中上调表达基因17个,下调表达基因3个。这些候选基因分别涉及不同水平分子进程和多种代谢途径。
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| 关 键 词： | 基因芯片 甘蔗 差异表达基因 |
Application of genechip in the study of diferentially expressed candidate genes in mature sugarcane leaves |
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| HUANG Feng,HE Wen-jin,DAI Rong-chun,LIN Rong-hua,YE Bing-ying,CHEN You-qiang.Application of genechip in the study of diferentially expressed candidate genes in mature sugarcane leaves[J].Subtropical Agriculture Research,2012,8(1):46-50. |
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| Authors: | HUANG Feng HE Wen-jin DAI Rong-chun LIN Rong-hua YE Bing-ying CHEN You-qiang |
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| Institution: | 1.College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350108,China;2.Key Lab of Fujian Sugarcane Biology and Genetic Breeding,Ministry of Agriculture,Fuzhou,Fujian 350002,China) |
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| Abstract: | In this study,Agilent sugarcane 4×44k(per array) genechip was used to identify the differentially expressed genes in the leaves of mature sugarcane,and bioinformatics tools were used for genechip hybridization analysis.Microarray hybridization results met the quality standards,the data showed excellent reproducibility and the results were reliable.The differentially expressed genes were screened from the large number genes based on the bioinformatics analysis and microarray by ratio value≥2 during the 8 periods of sugarcane growth.The common differentially expressed genes were 20 ESTs including 17 of Up-regulated and 3 of down-regulated ESTs genes.These genes involved in different molecular processes and a variety of metabolic pathways. |
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| Keywords: | genechip sugarcane differentially expressed gene |
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