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| 化学发光法测定烟叶中的总氮 | |
| 引用本文： | 徐宸,孙家利,张凤侠,王卫武,祖朝龙,时向东.化学发光法测定烟叶中的总氮[J].中国农学通报,2010,26(17):121-124. |
| 作者姓名： | 徐宸 孙家利 张凤侠 王卫武 祖朝龙 时向东 |
| 作者单位： | 1. 河南农业大学烟草行业栽培重点实验室,郑州,450002;重庆市烟草公司,重庆,400023 2. 河南农业大学烟草行业栽培重点实验室,郑州,450002 3. 河南农业大学资源与环境学院,郑州,450002 4. 安徽省农业科学院烟草研究所,合肥,230031 |
| 基金项目： | 国家烟草专卖局科技项目 |
| 摘 要： | 建立测定烟叶中总氮含量的新方法。烟叶中的含氮化合物经消化后，加碱蒸馏，用硫酸吸收，全部转化为氨态氮，根据NH4+在碱性溶液中能还原ClO ，该反应和化学发光反应ClO?-－Luminol相耦合，降低化学发光强度，从而达到测定NH4+的目的。本方法的检测下限达到10-3mg/kg，线性范围选择0.01～1.5（mg/kg），加标回收率在87.3%～109.9%之间，RSD＜5%(n=5)，完全能满足烟叶中总氮测定的要求。 |
| 关 键 词： | 文心兰 文心兰 原球茎 增殖 |
| 收稿时间： | 2009/3/13 0:00:00 |
| 修稿时间： | 2010/4/29 0:00:00 |
Determination of the Content of Total Nitrogen in Tobacco Leaveswith Chemiluminescence Method |
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| Xu Chen,Sun Jiali,Zhang Fengxia,Wang Weiwu,Zu Chaolong,Shi Xiangdong.Determination of the Content of Total Nitrogen in Tobacco Leaveswith Chemiluminescence Method[J].Chinese Agricultural Science Bulletin,2010,26(17):121-124. | |
| Authors: | Xu Chen Sun Jiali Zhang Fengxia Wang Weiwu Zu Chaolong Shi Xiangdong |
| Abstract: | In order to established a new method for determining the total nitrogen of tobacco, we studied the method of total nitrogen in tobacco by chemiluminescence. The tobacco leaves was digested, distilled with alkali by an improved distillation device, and absorbed by sulphuric acid, all the compounds which contain nitrogen in tobacco leaves change to ammonium nitrogen. NH4＋ can deoxidize ClO-in alkaline solution. This reaction couples with chemiluminescence reaction ClO--Luminol, which reduce the strength of luminosity. So that the content of the NH4＋is determined. The lower limit of detection reaches to 10-3 mg/kg. The choice of the line range is from 0.01 mg/kg to 1.5 mg/kg. The recovery of the method was 87.3%-109.9%. We measured the total nitrogen of the 14 tabacco samples by this method, We compared the results with Kjeldahl Nitrogen Method, RSD 5% （n = 5）, These conditions are completely contented with the requirement of determination of the total nitrogen in tobacco. |
| Keywords: | chemiluminescence tobacco leaves total nitrogen |
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