| 苎麻果胶合成重要酶UGlcAE基因的克隆及组织表达 |
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| 引用本文： | 刘建新,喻春明,唐守伟,朱爱国,王延周,朱四元,马雄风,熊和平.苎麻果胶合成重要酶UGlcAE基因的克隆及组织表达[J].作物学报,2008,34(11):1938-1945. |
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| 作者姓名： | 刘建新 喻春明 唐守伟 朱爱国 王延周 朱四元 马雄风 熊和平 |
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| 作者单位： | 中国农业科学院麻类研究所，湖南长沙 420105 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 以苎麻Boehmeria nivea (L.) Gaud]中苎1号为材料，采用简并RT-PCR法、RACE技术以及全长cDNA文库筛选，克隆苎麻果胶主要多糖组分的合成关键酶UGlcAE cDNA序列，序列长度为1 257 bp，编码区长723 bp，可编码241个氨基酸序列。实时定量PCR证实，UGlcAE表达量为根部＞叶片＞韧皮部＞木质部，在根部的表达量占优势。该结果对我们后期采用分子生物学方法来调控果胶的合成量具有实际意义。
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| 关 键 词： | 苎麻 果胶 UGlcAE 克隆 实时定量PCR |
| 收稿时间： | 2008-02-01 |
Cloning and Tissue Expression of Important Enzyme Gene UGlcAE in Ramie Pectin Biosynthesis |
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| LIU Jian-Xin,YU Chun-Ming,TANG Shou-Wei,ZHU Ai-Guo,WANG Yan-Zhou,ZHU Si-Yuan,MA Xiong-Feng,XIONG He-Ping.Cloning and Tissue Expression of Important Enzyme Gene UGlcAE in Ramie Pectin Biosynthesis[J].Acta Agronomica Sinica,2008,34(11):1938-1945. |
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| Authors: | LIU Jian-Xin YU Chun-Ming TANG Shou-Wei ZHU Ai-Guo WANG Yan-Zhou ZHU Si-Yuan MA Xiong-Feng XIONG He-Ping |
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| Institution: | Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 420105, Hunan, China |
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| Abstract: | Ramie Boehmeria nivea (L.) Gaud] is one of the important fibre crops, but its deguming leads to severe environmental pollution, large energy consumption, and deteriorated fibre for spinning and so on. Pectin is one of the primary colloid matter in ramie bast, and UGlcAE gene is a key enzyme gene in biosynthesis of most polysaccharide component of pectin. To inhibit pectin biosynthesis by antisense RNA or RNAi technology, some important genes such as UGlcAE related to pectin biosynthesis must be researched firstly. In this study, the full-length sequence of UGlcAE gene was cloned successfully from ramie cultivar-Zhongzhu 1 using degenerate primer RT-PCR, RACE, and screening of full-length cDNA library. The cDNA sequence was 1 257 bp, with a 723 bp encoding region and encode 241 amino acids. The Real-time quantitative PCR analysis showed that UGlcAE mRNA accumulated abundantly most in root and UGlcAE expression in ramie tissues was root>leaf>bast>xylem. These results pave the way for regulating and controlling synthetic quantity of pectin by molecular biology method in our further research. |
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| Keywords: | UGlcAE |
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