| 无花果品种“中国紫果”离体培养与保存研究 |
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| 引用本文： | 王亮,王彩虹,田义轲,王辉.无花果品种“中国紫果”离体培养与保存研究[J].河北林果研究,2009,24(1):81-83. |
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| 作者姓名： | 王亮 王彩虹 田义轲 王辉 |
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| 作者单位： | 青岛农业大学,园艺学院,山东,青岛,266109 |
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| 基金项目： | 青岛农业大学校级重点课题 |
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| 摘 要： | 本研究对无花果品种“中国紫果”的离体培养再生体系进行了探讨。结果表明，茎尖增殖的培养基为MS＋6-BA0.5mg／L＋NAA0.05mg／L，生根培养基为1／2MS＋IBA 0.2mg／L。将生根的无菌苗进行驯化后移栽到营养钵中，成活率可达100％。试验还表明GA3对“中国紫果”组培苗茎的伸长生长有显著效应。另外，试验探讨了“中国紫果”离体种质保存的限制生长保存方法，结果发现在1／2MS培养基（1．5％蔗糖）中，新梢基本无生长，7个月后转入含0．2mg／LGA3的增殖培养基中能迅速恢复生长。可见，用此方法可达到较好的短期保存效果。
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| 关 键 词： | 无花果 离体培养 离体保存 |
Propagation and conservation of 'Zhongguoziguo'(Ficus carica L.) |
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| WANG Liang,WANG Cai-hong,TIAN Yi-ke,WANG Hui.Propagation and conservation of 'Zhongguoziguo'(Ficus carica L.)[J].Hebei Journal of Forestry and Orchard Research,2009,24(1):81-83. |
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| Authors: | WANG Liang WANG Cai-hong TIAN Yi-ke WANG Hui |
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| Institution: | College of Horticulture;Qingdao Agricultural University;Qingdao 266109;China |
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| Abstract: | The propagation in vitro system of fig variety 'Zhongguoziguo' was studied.The result showed that the most suitable medium for proliferation was MS+ 0.5mg/L 6-BA +0.05 mg/L NAA and that for shoot rooting was 1/2MS+0.2 mg/L IBA.Plants were obtained from rooted plantlets after acclimatization and transplanting into pots.Meanwhile,it also indicated that GA3 had significant effect on the shoots growth and 0.2% GA3 could accelerate the elongation of the shoots distinctly.Furthermore,the conservation method of th... |
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| Keywords: | fig in vitro culture in vitro conservation |
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