| 藜麦叶片黄酮类物质的提取及基因型差异 |
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| 引用本文： | 陆敏佳,蒋玉蓉,陈国林,毛 前,陆国权.藜麦叶片黄酮类物质的提取及基因型差异[J].浙江农林大学学报,2014,31(4):534-540. |
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| 作者姓名： | 陆敏佳 蒋玉蓉 陈国林 毛 前 陆国权 |
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| 作者单位： | 1. 浙江农林大学 农业与食品科学学院，浙江 临安 311300；2. 浙江水利水电学院 水利工程系，浙江 杭州 310018 |
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| 基金项目： | 国家自然科学基金资助项目(31301372);浙江省重大科技专项计划项目(2011C12030);浙江农林大学科研发展基金资助项目(2012FK001) |
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| 摘 要： | 为了优化藜麦Chenopodium quinoa叶片黄酮的乙醇提取工艺和分析基因型间的差异,为藜麦黄酮的开发和高黄酮的品种筛选提供理论依据,采用3因子3水平正交试验设计,探讨了乙醇体积分数、料液比和浸提时间等因素对藜麦叶片黄酮提取率的影响;并采用最佳提取条件,对10个不同基因型品种藜麦的叶片黄酮得率进行了比较分析。结果表明:藜麦叶片黄酮最佳提取条件为体积分数70%乙醇,1∶40料液比,80℃水浴下回流浸提0.5 h。在优化条件下,1次提取工艺得率达85%以上。各因素对叶片黄酮提取率的影响程度依次为:浸提时间>乙醇体积分数>料液比。比较结果发现:藜麦叶片黄酮得率存在明显基因型差异,其中品种PI814932的叶片黄酮类物质得率最高,达0.933%。所测藜麦品种的叶片黄酮平均得率为0.619%,变异系数为34.44%。研究表明:乙醇回流法适于提取藜麦总黄酮类化合物。
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| 关 键 词： | 植物学 藜麦 黄酮 提取条件 基因型差异 |
| 收稿时间： | 2013-10-13; |
Flavonoid extraction and flavonoid content with genotypic variation from Chenopodium quinoa leaves |
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| LU Minjia,JIANG Yurong,CHEN Guolin,MAO Qian,LU Guoquan.Flavonoid extraction and flavonoid content with genotypic variation from Chenopodium quinoa leaves[J].Journal of Zhejiang A&F University,2014,31(4):534-540. |
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| Authors: | LU Minjia JIANG Yurong CHEN Guolin MAO Qian LU Guoquan |
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| Institution: | 1. School of Agricultural and Food Science, Zhejiang A & F University, Lin’an 311300, Zhejiang, China；2. Department of Hydraulic Engineering, Zhejiang University of Water Resources and Electric Power, Hangzhou 310018, Zhejiang, China） |
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| Abstract: | The suitable technology of extracting flavonoids from Chenopodium quinoa leaves using the ethanol reflux extraction method, as well as the analysis of flavonoid content with genotypic variation, were studied in the paper. The research aims to provide theoretical basis for flavonids exploitation from C. quinoa and high-flavonoid variety screening. The effect of each factor such as ethanol concentration, extracting time and ratio of sample with ethanol solution on flavonoid extraction from C. quinoa was investigated using the orthogonal design L9（33） test. Moreover, flavonoids from C. quinoa leaves of ten different genotypes were extracted by the optimum extraction method and were subsequently analyzed for averages and coefficient of variation （CV）. Results showed that optimum extracting conditions consisted of an extracting reagent of 70% ethanol, an extracting temperature of water-based heating of 80 ℃, a ratio of the leaf power to reagent of 1∶40, and an extraction time of 0.5 h. For the extraction rate of falvonoids from C. quinoa leaves extracting time＞ethanol concentration＞ratio of sample to the ethanol solution. In one extraction cycle with optimum extracting conditions, the flavonoid extraction rate reached more than 85%. Additionally, extraction yields of flavonoids from different genotypes different with cultivar ‘PI814932’ having the highest flavonoid extraction rate （0.933%）. The average extraction rate of flavonids from leaves of the ten tested varieties was 0.619%, and the CV was up to 34.44%. Hence, the ethanol reflux extraction method was suitable for flavonoid extraction from C. quinoa leaves. ［Ch, 4 tab. 29 ref.］ |
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| Keywords: | botany Chenopodium quinoaChenopodium quinoa flavonoid extraction conditions genotype variation |
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