| 4种竹子竹叶黄酮质量分数及抗氧化活性的季节变化 |
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| 引用本文： | 潘佳佳,叶生月,丁东栋,金 政,杨 萍.4种竹子竹叶黄酮质量分数及抗氧化活性的季节变化[J].浙江农林大学学报,2014,31(2):280-284. |
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| 作者姓名： | 潘佳佳 叶生月 丁东栋 金 政 杨 萍 |
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| 作者单位： | 1.浙江农林大学 林业与生物技术学院, 浙江 临安 3113002.浙江农林大学 亚热带森林培育国家重点实验室培育基地, 浙江 临安 3113003.桐庐县林业技术推广中心站, 浙江 桐庐 311500 |
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| 基金项目： | 杭州市科技发展计划项目(20101032B58);国家级大学生创新创业训练计划项目(201210341006);浙江省大学生科技创新活动计划(新苗人才计划)项目(2012R412006) |
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| 摘 要： | 以毛竹Phyllostachys edulis, 雷竹Phyllostachys violascens, 石竹Phyllostachys nuda和高节竹Phyllostachys prominens等4种竹子为研究对象, 用硝酸铝-亚硝酸钠比色法测定黄酮质量分数, 并采用DPPH·(1, 1-二苯基-2-三硝基苯肼)法和ABTS+·2, 2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐]法测定竹叶黄酮的抗氧化活性, 以考察竹叶黄酮质量分数和抗氧化活性随季节和竹种的变化规律。结果表明:春季毛竹的竹叶黄酮质量分数最高(2.12%±0.15%)(P<0.05), 夏季、秋季和冬季4种竹子间无显著性差异(P>0.05);石竹的竹叶黄酮质量分数四季变化不显著(P>0.05), 毛竹夏季的竹叶黄酮质量分数(2.36%±0.60%)高于冬季(1.52%±0.04%)(P<0.05), 雷竹(2.40%±0.17%)和高节竹(2.07%±0.13%)的竹叶黄酮质量分数以夏季最高。4种竹子的竹叶黄酮对DPPH·的清除能力都表现在夏季较强, 四季的各竹种竹叶黄酮对DPPH·的清除能力以毛竹较强。4种竹子的竹叶黄酮对ABTS+·的清除能力都表现在春季和秋季较强, 四季各竹种间差异性不大(P>0.05)。
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| 关 键 词： | 植物生理学 竹叶黄酮 DPPH· 清除能力 ABTS+· 清除能力 抗氧化活性 四季变化 |
| 收稿时间： | 2013-04-24; |
Seasonal flavonoid and anti-oxidant changes in leaves of four bamboo species |
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| PAN Jiajia,YE Shengyue,DING Dongdong,JIN Zheng,YANG Ping.Seasonal flavonoid and anti-oxidant changes in leaves of four bamboo species[J].Journal of Zhejiang A&F University,2014,31(2):280-284. |
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| Authors: | PAN Jiajia YE Shengyue DING Dongdong JIN Zheng YANG Ping |
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| Institution: | 1.School of Forestry and Biotechnology, Zhejiang A & F University, Lin'an 311300, Zhejiang, China2.The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin'an 311300, Zhejiang, China3.Forest Technology Extension Central Station of Tonglu County, Tonglu 311500, Zhejiang, China |
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| Abstract: | Leaves of four bamboo species: Phyllostachys edulis, Phyllostachys violascens, Phyllostachys nuda, and Phyllostachys prominens were studied to determine variation in flavonoid content and anti-oxidant activity for different seasons. Aluminum nitrate-sodium nitrite colorimetry was used to find the flavonoid content with 2,2 diphenyl-1-picrylhydrazyl（DPPH·） and 2,2-azino-bis-3-ethylbenzothiazoline-6- sulfonic acid（ABTS+·） being employed to discover anti-oxidative activity. Results in spring showed that flavonoid content of Ph. edulis was highest（2.12%±0.15%）（P＜0.05）, but in summer, autumn, and winter, there were no significant differences among the four bamboo species（P＞0.05） in the test flavonoid content. Flavonoid content of Ph. nuda did not change significantly （P＞0.05） during the four seasons; for Ph. edulis, summer flavonoid content（2.36%± 0.6%） was higher than winter（1.52%±0.04%）（P＜0.05）；and for Ph. violascens（2.40%±0.17%） and Ph. prominens （2.07%±0.13%） the highest flavonoid content was in summer. The scavenging ability of DPPH· for bamboo leaf flavonoids was higher in summer with Ph. edulis being best. The scavenging ability of ABTS+· for bamboo leaf flavonoids was greater in spring and autumn with no significance （P＞0.05） between species. These results can be used for the exploitation of bamboo resources. ［Ch, 6 fig. 12 ref.］ |
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| Keywords: | plant physiology flavonoids DPPH· ABTS+· anti-oxidant activities seasonal change |
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