| 恩诺沙星控制草鱼维氏气单胞菌的用药方案 |
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| 引用本文： | 王浩,权可艳,徐丽娟,李梦影,吕利群.恩诺沙星控制草鱼维氏气单胞菌的用药方案[J].淡水渔业,2013(2):48-53. |
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| 作者姓名： | 王浩 权可艳 徐丽娟 李梦影 吕利群 |
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| 作者单位： | 农业部淡水水产种质资源重点实验室上海海洋大学水产与生命学院;四川省水产学校 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(CARS-46-12);公益性行业(农业)科研专项经费“渔药使用风险评估及其控制技术研究与示范”(No.201203085) |
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| 摘 要： | 从四川成都一养殖场大规模发病死亡的草鱼(Ctenopharyngodon idella)病灶处分离一株病原菌CiAV01,结合形态学和16S rDNA序列分析,鉴定该菌为维氏气单胞菌(Aeromonas veronii)。回归感染实验结果表明,分离菌株感染草鱼致死率为100%,出现了局部出血、腹腔积水等症状。采用琼脂平板扩散法研究了16种抗生素对分离菌株CiAV01的体外抑菌作用,在供试的抗生素中,恩诺沙星对该分离菌株CiAV01最为敏感。选择恩诺沙星对分离菌株进行体外药效学研究表明,恩诺沙星对分离菌株CiAV01的最小抑菌浓度(MIC)为0.25μg/mL,最小杀菌浓度(MBC)为0.50μg/mL,MBC/MIC为2。结合其药物动力学参数和MIC、MBC、防突变浓度(MPC)、突变选择窗(MSW)和抗菌后效应(PAE)制定防突变给药方案为:剂量20 mg/kg、每日一次给药、连续给药3～5 d。
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| 关 键 词： | 维氏气单胞菌(Aeromonas veronii) 恩诺沙星 防突变浓度 草鱼(Ctenopharyngodon idella) |
Dosing guidelines of enrofloxacin in Ctenopharyngodon idella against Aeromonas veronii |
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| Institution: | WANG Hao1,QUAN Ke-yan2,XU Li-juan1,LI Meng-ying1,LU Li-qun1(1.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China; 2.College of Fisheries,Chengdu 611730,China) |
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| Abstract: | A pathogenic bacterical strain CiAV01 was isolated from naturally infected Ctenopharyngodon idella in a fish farm in Sichuan province.The strain CiAV01 was identified as Aeromonas veronii,according to its physiological and 16S rDNA sequence.Recurrent infection showed that the mortality of Ctenopharyngodon idella is 100% after artificial infect healthy Ctenopharyngodon idella with the symptoms of hemorrhage and ascites.The drug sensitivity of 16 antibiotics was studied using Kirby-Bauer's ager diffusion method,and enrofloxacin was the most highly sensitive to the strain CiAV01.The pharmacodynamics(PD) parameters of enrofloxacin against CiAV01 PK/PD indices are MIC 0.25 μg/mL,MBC 0.50 μg/mL,MBC/MIC 2,MPC,MPC 2 μg/mL and MSW 0.25~2.0 μg/mL,respectively.Based on pharmacokinetics/pharmacodynamics parameters,and MIC,MBC,MPC,MSW and PAE,once-daily dose of 20 mg/kg for 3~5 days was determined to be optimal for controlling Aeromonas veronii CiAV01 infection and simultaneously preventing mutant selection in grass carp based on pharmacokinetics/pharmacodynamics parameters and MIC,MBC,MPC,MSW,PAE. |
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| Keywords: | Aeromonas veronii enrofloxacin MPC Ctenopharyngodon idella |
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