| 草莓离体培养中玻璃化的发生及克服措施研究 |
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| 引用本文： | 丰锋,陈厝边.草莓离体培养中玻璃化的发生及克服措施研究[J].西南农业大学学报,2007,29(4):78-82. |
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| 作者姓名： | 丰锋 陈厝边 |
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| 作者单位： | 广东海洋大学农学院,广东湛江524088 |
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| 基金项目： | 广东省农业厅农业科技项目（B06026）. |
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| 摘 要： | 以“丰香”品种为试材,MS为基本培养基,研究草莓玻璃化的克服措施,结果表明,在6-BA试验浓度范围内（1-3 mg·L^-1）,玻璃化率随6-BA浓度的增加而增加;40 g·L^-1蔗糖,降低MS基本培养基中铵离子浓度,加入活性炭,采用透气膜封口在一定程度上均能使玻璃苗恢复正常.
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| 关 键 词： | 草莓 离体培养 玻璃化 |
| 文章编号： | 1000-2642（2007）04-0078-05 |
| 修稿时间： | 2006-10-31 |
Vitrification in in Vitro Culture of Strawberry and Its Prevention |
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| FENG Feng, CHEN Cuo-bian.Vitrification in in Vitro Culture of Strawberry and Its Prevention[J].Journal of Southwest Agricultural University,2007,29(4):78-82. |
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| Authors: | FENG Feng CHEN Cuo-bian |
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| Institution: | College of Agriculture, Guangdong Ocean University, Zhanjiang , Guangdong 524088, China |
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| Abstract: | Cultured in vitro on MS medium supplemented with 6-BA,the strawberry variety "Tochiotome" showed increasing vitrification rate with increasing 6-BA concentration(1~3 mg/L).The addition of sucrose to the medium at 40 g/L helped to decrease the number of vitreous plantlets.The measures of decreasing NH+4 concentration in the medium,or adding active carbon to it,or sealing the bottle with a permeable film helped the vitreous plantlets change back to normal ones. |
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| Keywords: | strawberry in vitro culture vitrification |
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