Starch characterisation and variability in GBSS loci of synthetic hexaploid wheats and their durum and <Emphasis Type="Italic">Aegilops tauschii</Emphasis> parents |
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| Authors: | C M Konik-Rose S Rahman R Appels R Moss G McMaster D R Marshall F L Stoddard |
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| Institution: | (1) CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia;(2) BRI Australia Ltd, P.O. Box 7, North Ryde, NSW, 1670, Australia;(3) Plant Breeding Institute, The University of Sydney, PMB 11, Camden, NSW, 2570, Australia;(4) Present address: Murdoch University, Murdoch, WA, 6150, Australia;(5) Present address: Allied Mills Australia, Summer Hill, NSW, 2130, Australia;(6) Present address: Cooks Hill, NSW, 2300, Australia;(7) Present address: Department of Applied Biology, University of Helsinki, P.O. Box 27, Helsinki, 00014, Finland |
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| Abstract: | Greater variability in starch properties is found in lower ploidy wheats than in commercial hexaploid wheats. This paper reports
on the starch properties and variability in granule bound starch synthase (GBSS) loci of 17 diploid (Aegilops tauschii) and 12 tetraploid (durums) potential progenitors of wheat, compared with 29 synthetic hexaploid wheats produced from such
progenitors. Starch properties examined were granule size distribution, swelling power, amylose content, gelatinisation and
amylose-lipid dissociation properties. A PCR screening method was able to detect the presence or absence of each of the three
GBSS genes. It also detected polymorphisms in eight diploids and nine hexaploids, all displaying the same 25 bases deletion
in the D genome allele of GBSS. Two tetraploids and five hexaploids were null 4A for GBSS. There was little difference in
the amylose contents and amylose-lipid dissociation peak temperatures of the synthetic hexaploids and the lower ploidy wheats.
The synthetic hexaploids showed intermediate swelling power values with the durums giving the highest swelling powers. The
durums also had higher B granule contents than the A. tauschii accessions, but not as high as the synthetics. However, the A. tauschii samples gave the highest gelatinisation peak temperatures. The presence of the null 4A mutation was positively correlated
with swelling power, amylose content and DSC measurements. The new smaller D genome allele of GBSS was associated with slightly
higher swelling power. These results confirm the value of wheat progenitor lines as sources of new starch properties for hexaploid
wheat.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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| Keywords: | Aegilops tauschii Durum Granule bound starch synthase Starch Synthetic wheat |
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