| 人工饲养蓝狐微卫星DNA的多态性 |
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| 引用本文： | 傅凯,白素英,金煜.人工饲养蓝狐微卫星DNA的多态性[J].东北林业大学学报,2010,38(2). |
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| 作者姓名： | 傅凯 白素英 金煜 |
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| 作者单位： | 国家林业局野生动植物检测中心(东北林业大学),哈尔滨,150040;国家林业局野生动植物检测中心(东北林业大学),哈尔滨,150040;国家林业局野生动植物检测中心(东北林业大学),哈尔滨,150040 |
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| 基金项目： | 黑龙江省科技攻关项目(GC03B515) |
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| 摘 要： | 选取16个微卫星位点对5个蓝狐群体进行遗传多态性研究,利用PopGen32和MEGA4对5个群体的等位基因数、有效等位基因数、等位基因频率、观测杂合度、期望杂合度和多态信息含量等遗传参数进行了分析。结果表明:5个群体137个个体共计检出162个等位基因,每个位点等位基因数为4～13。位点多态信息含量为0.17～0.84,5个群体中3个群体为高度多态性,其余2个群体为中度多态。位点的观测杂合度为0.18～0.89,位点的期望杂合度为0.18～0.86,茂兴湖群体的杂合程度均高于其他4个种群。选取的16个微卫星位点均表现出较高的多态性,在蓝狐多样性分析中具有一定的有效性,可用于蓝狐遗传多样性的分析。
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| 关 键 词： | 蓝狐 荧光标记引物 微卫星DNA多态性 |
Microsatellite DNA Polymorphism in Blue Fox |
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| Fu Kai,Bai Suying,Jin Yu.Microsatellite DNA Polymorphism in Blue Fox[J].Journal of Northeast Forestry University,2010,38(2). |
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| Authors: | Fu Kai Bai Suying Jin Yu |
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| Institution: | Fu Kai,Bai Suying,Jin Yu(State Forestry Administration Detecting Center of Wildlife(Northeast Forestry University),Harbin 150040,P.R.China) |
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| Abstract: | Sixteen microsatellite loci were used to investigate genetic diversity of five blue fox populations.Genetic parameters,including number of alleles,effective number of alleles,allele frequency,observed heterozygosity,expected heterozygosity,and polymorphism information content,were analyzed by PopGen32 and MEGA4.162 alleles were detected in 137 individuals from 5 populations,and the number of alleles per locus ranged from 4 to 13 and polymorphism information content was between 0.17 and 0.84.The populations ... |
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| Keywords: | Blue foxes Fluorescence labeling primers Microsatellite DNA polymorphism |
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