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Supplementation of tryptophan and lysine in Diplodus sargus larval diet: effects on growth and skeletal deformities |
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| Authors: | Margarida Saavedra Yoav Barr Pedro Pousão-Ferreira Synnove Helland Manuel Yúfera Maria T Dinis & Luís E C Conceição |
| Institution: | Instituto Nacional de Investigação Agrária e das Pescas (INIAP/IPIMAR-CRIPSul), Olhão, Portugal; Nofima Marin, Sunndalsora, Norway; Instituto de Ciencias Marinas Andalucia (CSIC), Puero Real, Spain; CCMAR, Universidade do Algarve, Campus Gambelas, Faro, Portugal |
| Abstract: | Amino acids are the building blocks for growth and the major energy source during fish larval stages. Deficient amino acids can be supplemented in the diets, overcoming problems such as low growth rates and skeletal deformities. In this study, three experimental diets were tested: a balance diet supplemented with lysine, a balance diet supplemented with tryptophan and a control with no supplementation. Trials were conducted with Diplodus sargus larvae from 1 to 25 days after hatching (DAH). A microencapsulated diet was introduced at 15 DAH in co-feeding with live feed and from 20 DAH larvae were fed only this diet. The effect of the supplemented diets was assessed in terms of survival, growth rate, skeletal deformities, ammonia excretion and activity of amino acid catabolism enzymes. The results showed a similar survival in all treatments. However, larvae given tryptophan supplementation had a lower weight on 25 DAH. No significant differences were found in ammonia excretion, frequency or type of deformities or enzymatic activity. Tryptophan and lysine supplementation failed to improve larval growth, survival or larval quality. |
| Keywords: | Diplodus sargus amino acids tryptophan lysine ammonia skeletal deformities growth |