| 二长棘犁齿鲷线粒体DNA控制区结构和进化 |
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| 引用本文： | 苏天凤,江世贵,马之明,李夏.二长棘犁齿鲷线粒体DNA控制区结构和进化[J].南方水产,2012,8(6):1-8. |
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| 作者姓名： | 苏天凤 江世贵 马之明 李夏 |
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| 作者单位： | 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东广州 510300 |
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| 摘 要： | 测定了二长棘犁齿鲷（Evynnis cardinalis）线粒体控制区全序列，并结合从GenBank中下载的9种鲷科鱼类的相应序列，采用ClustalX对控制区结构进行了分析，识别了相应的保守序列，包括终止相关序列ETAS、中央保守区的CSB-F、CSB-E、CSB-E、CSB-C、CSB-B和CSB-A序列，以及保守序列区的CSBl、CSB2和CSB3序列。以副鲈（Paralabrax clathratus）和条斑星鲽（Verasper moseri）为外群，用最大简约法（MP）和邻接法（NJ）构建了系统发育树。结果显示鲷科鱼类构成一个单系类群，二长棘犁齿鲷应该归入赤鲷属，建议有必要对犁齿鲷属和赤鲷属的分类地位重新评估。
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| 关 键 词： | 二长棘犁齿鲷 控制区 结构 系统关系 |
Structure and evolution of mitochondrial control regions in Evynnis cardinalis |
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| SU Tianfeng
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JIANG Shigui
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MA Zhiming
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LI Xia.Structure and evolution of mitochondrial control regions in Evynnis cardinalis[J].South China Fisheries Science,2012,8(6):1-8. |
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| Authors: | SU Tianfeng JIANG Shigui MA Zhiming LI Xia |
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| Institution: | (Key Lab. of South China Sea Fisheries Resources Exploitation ＆ Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China) |
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| Abstract: | We determined the complete sequences of the mitochondrial DNA control regions in Evynnis cardinalis using long polymer- ase chain reaction （PCR）, and aligned them by Clustal X with 9 related Sparidae sequences in GenBank. Several conserved sequence blocks were identified in the mitochondrial DNA control regions, including extended terminal associated sequences （ETAS）, blocks of central domain （CSB-F, CSB-E, CSB-D, CSB-C, CSB-B, CSB-A） and conserved sequence blocks （CSB1, CSB2, CSB3）. Tak- ing Paralabrax clathratus and Verasper moseri as outgroups, the molecular phylogenetic trees of Sparidae were constructed based on neighbour-joining and maximum-parsimony methods, which reveal that Sparidae is a monophyletic group, and E. cardinalis should be grouped with Pagrus. Therefore, it is necessary to reevaluate the taxonomic status of Evynnis and Pagrus. |
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| Keywords: | Evynnis cardinalis control region structure phylogeny |
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