| 酢浆草"熔岩"的组培快繁技术研究 |
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| 引用本文： | 陈芬,金倩,周玲玲,张黎杰,谭军.酢浆草"熔岩"的组培快繁技术研究[J].青岛农业大学学报(自然科学版),2017(3):200-204. |
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| 作者姓名： | 陈芬 金倩 周玲玲 张黎杰 谭军 |
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| 作者单位： | 江苏省农业科学院宿迁农科所,江苏 宿迁,223800 |
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| 基金项目： | 宿迁市农业科技自主创新项目(SQCX2015-02) |
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| 摘 要： | 本研究选取"熔岩"酢浆草茎为外植体进行了组织培养研究,建立了其再生快繁体系.结果表明,适合丛生芽诱导及增殖的培养基是MS+0.2 mg/LNAA+1 mg/L6-BA,丛生芽诱导率可达100%,增殖系数达35.11;适合壮苗培养基是不添加激素的MS培养基,最佳生根培养基为1/2MS+0.05 mg/LNAA,平均根数达4.73,平均根长达3.75.为"熔岩"酢浆草的大规模生产提供基础数据.
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| 关 键 词： | "熔岩"酢浆草 组培 快繁 激素 |
Study on Tissue Culture and Rapid Propagation System of Oxalis Sunset Velvet |
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| CHEN Fen,JIN Qian,ZHOU Lingling,ZHANG Lijie,TAN Jun.Study on Tissue Culture and Rapid Propagation System of Oxalis Sunset Velvet[J].Journal of Laiyang Agricultural College,2017(3):200-204. |
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| Authors: | CHEN Fen JIN Qian ZHOU Lingling ZHANG Lijie TAN Jun |
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| Abstract: | The stems of Oxalis Sunset Velvetl were used as explants in tissue culture and rapid propagation system.The results showed that the optimal medium for inducing clustered shoots was MS + 0.2 mg/LNAA + 1 mg/L6-BA with 100% induction rate and 35.11 multiplication coefficient.The suitable medium for strong seedling was MS without hormones.The optimal medium for rooting was 1/2 MS + 0.05 mg/LNAA.Average root number and average root length were 4.73 and 3.75 respectively.The results indicated that the Oxalis Sunset Velvet was able to provide the basic data for mass production. |
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| Keywords: | Oxalis Sunset Velvet tissue culture rapid propagation hormone |
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