| 两种桫椤属植物组培快繁技术研究 |
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| 引用本文： | 郎月婷,马长旺,于靖,郭芮,严岳鸿,杨东梅.两种桫椤属植物组培快繁技术研究[J].热带作物学报,2021,42(6):1579-1588. |
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| 作者姓名： | 郎月婷 马长旺 于靖 郭芮 严岳鸿 杨东梅 |
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| 作者单位： | 1. 海南大学园艺学院,海南海口 5702282. 海南省热带园艺作物品质调控重点实验室,海南海口 5702283. 上海辰山植物园/中国科学院上海辰山植物科学研究中心,上海 201602 |
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| 基金项目： | 上海市绿化和市容管理局科研项目(G182411);海南省创新科研课题(Hys2019-215) |
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| 摘 要： | 以阴生桫椤（Alsophila latebrosa）、大叶黑桫椤（Alsophila gigantea）为研究对象,利用绿色球状体（Green Globular Bodies,GGB）途径,展开快繁体系的研究,以筛选最优的繁殖体系。结果表明：（1）阴生桫椤的快繁体系中,1/10 MS是孢子萌发最优培养配方;1/2 MS+NAA 0.3 mg/L+6-BA 0.5 mg/L,蔗糖30 g/L,琼脂6.5 g/L是诱导配子体产生GGB的最优培养配方;1/2 MS+KT 0.1 mg/L+IAA 0.1 mg/L是其诱导GGB分化的最优培养配方;孢子体幼苗在泥炭土:菜园土:珍珠岩=3:3:1的基质中成活率最高。（2）在大叶黑桫椤的快繁体系中,1/10 MS为相对较好的培养基;1/2 MS+0.3 mg/L IBA是诱导产生GGB的最优培养配方;1/2 MS+0.3 mg/L NAA对GGB分化的促进作用最显著;1/2 MS+0.1 mg/L IAA+0.3 mg/L NAA的组合可有效促进孢子体幼苗生根。（3）通过配子体诱导GGB途径,2种桫椤属植物从孢子萌发到幼苗移栽的整个培养过程为6~8个月。可见,GGB途径不仅能提高繁殖系数,也能大大缩短桫椤属植物的繁殖时间,为实现桫椤属植物的工厂化育苗奠定了基础。
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| 关 键 词： | 阴生桫椤 大叶黑桫椤 GGB 组织培养 |
| 收稿时间： | 2020-05-19 |
Technology for Rapid Propagation in Vitro of Two Alsophila Species |
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| LANG Yueting,MA Changwang,YU Jing,GUO Rui,YAN Yuehong,YANG Dongmei.Technology for Rapid Propagation in Vitro of Two Alsophila Species[J].Chinese Journal of Tropical Crops,2021,42(6):1579-1588. |
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| Authors: | LANG Yueting MA Changwang YU Jing GUO Rui YAN Yuehong YANG Dongmei |
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| Institution: | 1. College of Horticulture, Hainan University, Haikou, Hainan 570228, China2. Key Laboratory for Quality Regulation of Tropical Horticultural Plants of Hainan Province, Haikou, Hainan 570228, China3. Shanghai Chenshan Botanical Garden / Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602, China |
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| Abstract: | Alsophila latebrosa and A. gigantea both have high scientific value and ornamental value. The construction of the rapid propagation system of them by gametophy-induced Green Globular Bodies (GGB) pathway was studied. In the rapid propagation system of A. latebrosa, the most optimal culture formula for spore germination was 1/10MS, the best culture medium for inducing gametophytes to produce GGB was 1/2MS+NAA 0.3 mg/L+6-BA 0.5 mg/L, sucrose 30 g/L and agar 6.5 g/L, the most suitable formula for GGB differentiation factor was 1/2MS+KT 0.1 mg/L+NAA 0.1 mg/L, and the survival rate of sporophyte seedlings was the highest in the substrate (peat:garden soil:perlite = 3:3:1). In the fast propagation system ofA. gigantea,the relatively suitable medium was 1/10MS, the most optimal formula for GGB induction was 1/2MS+0.3 mg/L IBA, NAA of 0.3 mg/L had the most significant effect on GGB differentiation, the combination of 0.1 mg/L IAA+0.3 mg/L NAA could promote the rooting the sporophyte seedlings. It only took 6-8 months by gametophy-induced GGB pathway for the culture of the two Alsophilaspecies, from spore germination to seeding transplanting. Therefore, this pathway not only increased the propagation coefficiency, but also greatly reduced the propagation time of Alsophila species, which could enhance the industrial seeding of Alsophilaspecies. |
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| Keywords: | Alsophila latebrosa Alsophila gigantea green globular bodies tissue culture |
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