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茉莉花白绢病露天接种技术及盆栽抗病性鉴定
引用本文:李春牛,李先民,杜婵娟,孙明艳,黄展文,卢家仕,付岗,卜朝阳.茉莉花白绢病露天接种技术及盆栽抗病性鉴定[J].热带作物学报,2021,42(8):2350-2355.
作者姓名:李春牛  李先民  杜婵娟  孙明艳  黄展文  卢家仕  付岗  卜朝阳
作者单位:1.广西农业科学院花卉研究所,广西南宁 5300072.广西农业科学院植物保护研究所,广西南宁 530007
基金项目:广西重点研发计划项目(桂科AB18221064);广西科技基地和人才专项(桂科AD17195065);广西农业科学院科技发展基金资助项目(桂农科2021JM29)
摘    要:为了鉴定茉莉花资源对白绢病的抗性,从主产区广西横县的病株分离白绢病菌株,以横县双瓣茉莉花为材料,对露天条件下茉莉花白绢病接种方法进行了研究,并对盆栽的不同茉莉花品种(系)进行接种,采用相对抗病程度评价法鉴定供试材料的抗病性。结果表明:(1)在5种接种方法中,带菌燕麦粒埋土法的病情指数、发病率最高,分别为47.93%、86.67%,可以作为茉莉花白绢病露天接种方法;以此法接种后0~24 d期间,随着苗龄增加,发病率及病情指数呈降低趋势,1年生盆栽发病最迅速,病情指数也一直最高,20年生的发病最慢,病情指数一直最低;(2)供试的18份茉莉花品种(系)中,有抗病品种(系)5个、中感品种(系)6个、高感品种(系)7个,其中J0718的相对抗性指数最高,虎头茉莉和J1836最容易感染茉莉花白绢病,横县双瓣茉莉属中感品种;(3)供试的其他8份素馨属资源中,迎春花的相对抗性指数为0.93,为高抗品种;抗病品种4个,包括耳叶茉莉、扭肚藤、厚叶素馨及浓香茉莉,高抗及抗病品种占62.50%,可为茉莉花抗性育种提供种质资源;(4)在抗病及高感种质资源中,都有单瓣、双瓣及多瓣类型,不宜仅依据瓣型来判断抗性强弱。

关 键 词:茉莉花  白绢病  接种技术  抗性鉴定  
收稿时间:2020-11-16

Artificial Inoculation Technique for Jasmine Southern Blight Caused by Sclerotium rolfsii and Evaluation Resistance of Potted Jasminum sambac
LI Chunniu,LI Xianmin,DU Chanjuan,SUN Mingyan,HUANG Zhanwen,LU Jiashi,FU Gang,BU Zhaoyang.Artificial Inoculation Technique for Jasmine Southern Blight Caused by Sclerotium rolfsii and Evaluation Resistance of Potted Jasminum sambac[J].Chinese Journal of Tropical Crops,2021,42(8):2350-2355.
Authors:LI Chunniu  LI Xianmin  DU Chanjuan  SUN Mingyan  HUANG Zhanwen  LU Jiashi  FU Gang  BU Zhaoyang
Institution:1. Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China2. Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China
Abstract:In order to identify the resistance of jasmine resources to Sclerotium rolfsii, a strain of S. rolfsii was isolated from Jasminum sambac grown in Hengxian, and the inoculation technology of the strain was explored with Hengxian double petal jasmine as the material, and the resistance of different varieties of potted jasmine by relative disease resistance was evaluated. The disease index and incidence rate of the oat seed embedding method were the highest, being 47.93 and 86.67% respectively, which could be used as the outdoor inoculation method for the jasmine southern blight. 0-24 d after inoculation, the incidence and disease index decreased with the increase of seedling age, and the incidence rate of the plant which was 1 year old was the fastest and the disease index was the highest. The incidence of the plant which was 20 years old was the slowest and the disease index was the lowest. There were 5 varieties that were resistant to the disease, 6 varieties were medium susceptible varieties and 7 varieties were high susceptible varieties. The relative resistance index of J0718 was the highest. J. sambac ‘Grand Duke of Tuscany’ and J1836 were the most susceptible to jasmine southern blight, and Hengxian double petal jasmine was medium susceptible variety. The other 8 Jasmine resources from Jasminum L were tested. J. Nudiflorum was a high resistance variety with a relative resistance index of 0.93. Four were resistant varieties, including J. auriculatum, J. elongatum, J. pentaneurum and J. odoratum. 62.50% of the 8 varieties were high resistance and disease resistance varieties, which could be used for jasmine resistance breeding. In the disease resistant and high sensitive germplasm resources, there were single, double and multi valve types. The resistance to jasmine southern blight was not related to the corolla type.
Keywords:Jasminum sambac   Sclerotium rolfsii  artificial inoculation technique  evaluation of resistance  
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