| 不同方法提取的鹧鸪茶多酚抗氧化及抑菌性比较 |
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| 引用本文： | 段宙位,李鹏,何艾,王世萍,谢辉.不同方法提取的鹧鸪茶多酚抗氧化及抑菌性比较[J].热带作物学报,2021,42(3):847-853. |
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| 作者姓名： | 段宙位 李鹏 何艾 王世萍 谢辉 |
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| 作者单位： | 1.海南省农业科学院农产品加工设计研究所,海南海口 5711002.华中农业大学食品科技学院,湖北武汉 4300703.海南大学动物科技学院,海南海口 570228 |
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| 基金项目： | 海南省自然科学基金项目(No.319QN328);2018年海南省农业科学院农业科技创新专项项目。 |
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| 摘 要： | 为筛选适合鹧鸪茶多酚的提取方法,在热水浸提法、有机溶剂浸提法和纤维素酶辅助浸提法提取鹧鸪茶多酚基础上,采用DPPH自由基法、ABTS自由基法、羟自由基法、超氧阴离子法和还原力法评价多酚提取物的抗氧化性;滤纸片扩散法和二倍稀释法测定多酚提取物的抑菌性。结果表明,热水浸提法、有机溶剂浸提法和纤维素酶辅助浸提法提取鹧鸪茶多酚的得率分别为(10.31±0.42)%、(9.79±0.38)%、(10.88±0.52)%;含量分别为(44.72±2.26)%、(39.54± 1.78)%、(47.07±2.52)%。3种方法浸提的多酚提取物均具有较强的抗氧化性,纤维素酶辅助提取的鹧鸪茶多酚（CFC）清除DPPH自由基能力最强,其半抑制浓度（IC50）为(0.0034±0.0002)mg/mL;热水浸提的鹧鸪茶多酚（WFC）清除ABTS自由基、羟自由基和超氧阴离子自由基能力最强,其IC50值分别为(0.066±0.004)、(0.069±0.004)、(0.127± 0.009)mg/mL。3种方法浸提的多酚提取物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、绿脓杆菌均有较好抑制作用,其中WFC对4种细菌的抑制效果最好,抑菌圈直径依次为(12.34±1.01)、(12.16±0.95)、(2.12±0.15)、(6.12±0.36)mm,最小抑菌浓度（minimum inhibitory concentration,MIC）依次为3.13、3.13、12.50、6.25 mg/mL。综合考虑,鹧鸪茶多酚提取采用热水浸提法较为适宜。
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| 关 键 词： | 鹧鸪茶 多酚 提取 抗氧化活性 抑菌性 |
| 收稿时间： | 2020-03-28 |
Antioxidant and Bacteriostasis Activity of Flavanoid from Mallotus oblongifolius by Different Extraction Methods |
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| DUAN Zhouwei,LI Peng,HE Ai,WANG Shiping,XIE Hui.Antioxidant and Bacteriostasis Activity of Flavanoid from Mallotus oblongifolius by Different Extraction Methods[J].Chinese Journal of Tropical Crops,2021,42(3):847-853. |
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| Authors: | DUAN Zhouwei LI Peng HE Ai WANG Shiping XIE Hui |
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| Institution: | 1. Institute of Processing & Design of Agroproducts, Hainan Academy of Agricultural Science, Haikou, Hainan; 571100, China2. Collage of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China3. College of Animal Science and Technology, Hainan University, Haikou, Hainan 570228, China |
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| Abstract: | The aim was to select the suitable method for extracting the polyphenols from Mallotus oblongifolius.The polyphenols were extracted by hot water extraction,organic solvent extraction and cellulase-assisted extraction.The antioxidant activity of the extract density was evaluated by scavenging DPPH,ABTS,hydroxyl,superoxide anion radical and reducing force methods.The antibacterial activity of the polyphenol extracts was determined by filter paper diffusion method and double dilution methods.The yield of polyphenols by the hot water extraction method,organic solvent extraction method and cellulase-assisted extraction method was(10.31±0.42)%,(9.79±0.38)%,and(10.88±0.52)%,respectively,and the content was(44.72±2.26)%,(39.54±1.78)%,and(47.07±2.52)%.The polyphenol extracts extracted by the three methods had strong antioxidant properties.The cellulose-assisted extraction of the polyphenols from M.oblongifolius(CFC)had the strongest ability to scavenge DPPH radical,and its half inhibitory concentration(IC50)was(0.0034±0.0002)mg/mL.The hot water-extracted polyphenols from M.oblongifolius(WFC)had the strongest ability to scavenge ABTS radical,hydroxyl radical,and superoxide anion radical,and the IC50 values was(0.066±0.004),(0.069±0.004),(0.127±0.009)mg/mL.The polyphenol extracts extracted by the three methods had a good inhibitory effect on four bacteria.Among them,WFC had a good inhibitory effect on Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Pseudomonas aerugino,the inhibitory zone diameter was(12.34±1.01),(12.16±0.95),(2.12±0.15),(6.12±0.36)mm,and the minimum inhibitory concentration(MIC)was 3.13,3.13,12.50,6.25 mg/mL.Comprehensive consideration,the hot water extraction method is more suitable for the extraction of polyphenols from M.oblongifolius. |
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| Keywords: | Mallotus oblongifolius flavonoids extraction antioxidant activity antibacterial |
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