| 牦牛谷胱甘肽过氧化酶1基因(GPX1)的克隆及系统发育分析 |
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| 引用本文： | 李瑞哲,徐惊涛,马志杰,孙永刚,韩银仓,陈生梅.牦牛谷胱甘肽过氧化酶1基因(GPX1)的克隆及系统发育分析[J].青海畜牧兽医杂志,2021(1):1-5. |
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| 作者姓名： | 李瑞哲 徐惊涛 马志杰 孙永刚 韩银仓 陈生梅 |
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| 作者单位： | 青海大学畜牧兽医科学院 |
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| 基金项目： | 青海省畜牧兽医科学院基本科研业务费自主选题项目(MKY-2019-07)。 |
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| 摘 要： | 本研究克隆了牦牛谷胱甘肽过氧化酶1GPX1基因的CDS区序列,分析了其核苷酸序列,并进行了系统发育分析。结果表明,牦牛GPX1基因CDS区全长618 bp,编码205个氨基酸;经与GenBank中其他物种GPX1基因CDS区比对,牦牛GPX1基因CDS区与普通牛和瘤牛完全一致,与水牛、绵羊和猪的序列一致性较高,与其他哺乳动物序列一致性较低。本研究为深入研究牦牛GPX1的生理功能提供了参考资料。
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| 关 键 词： | 牦牛 谷胱甘肽过氧化酶1 基因克隆 系统发育 |
Cloning and Phylogenetic Analysis of Glutathione Peroxidases1 Gene(GPX1)in Yak |
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| Institution: | (Academy of Animal Science and Veterinary Medicine of Qinghai University, Xining 810016) |
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| Abstract: | Glutathione Peroxidases1,which is the most abundantly expressed enzymes of glutathione peroxidases family,plays an important role in inactivating peroxides(hydrogenperoxides,organic hydroperoxides and lipid peroxides et al.)and sustaining cellular re-dox homeostasis.In this study,we cloned the coding sequence region(CDS)of GPX1 gene in yak,analyzed its nucleotide sequence and constructed phylogenetic treeusing bioinformatic software.The results showed that the CDS of yak GPX1 gene spans 618 bp,which encodes 205 amino acids.The aligning results showed that the CDS of yak GPX1 gene had an identical sequence with cattle and zebu,a high similarity with water buffalo,sheep and pig,and a lower similarity with other mammals.This study provided basic reference data for further investigating the biological role of GPx1 in yak. |
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| Keywords: | Yak Glutathione peroxidase1 Gene cloning Phylogenetic analysis |
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