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金花茶组叶片生化成分评价及其最佳采收期研究
引用本文:刘保财,陈菁瑛,张武君,黄颖桢,赵云青.金花茶组叶片生化成分评价及其最佳采收期研究[J].热带作物学报,2021,42(1):159-167.
作者姓名:刘保财  陈菁瑛  张武君  黄颖桢  赵云青
作者单位:1.福建省农业科学院农业生物资源研究所,福建福州 3500032.福建省农业科学院药用植物研究中心,福建福州 350003
基金项目:福建省农业科学院项目(No.A2016-1);福建省财政专项“福建省农业科学院药用植物创新团队建设”项目(No.STIT2017-2-8);福建省农业科学院生产性工程化实验室建设项目(No.2015GCH-6)。
摘    要:本研究以26份来源于6种金花茶组植物、不同表型防城金花茶和不同月份采收的防城金花茶的成熟叶片为材料,建立同时测定芦丁、槲皮素、木犀草素、山奈酚4种黄酮成分的HPLC法,以及优化了测定茶多酚、总黄酮、总多糖和总皂苷成分含量的紫外分光光度法,基于以上8种成分含量,结合主成分综合评分和聚类分析的化学计量学方法,对不同来源金花...

关 键 词:金花茶组  多成分测定  化学计量学  品质评价  采收期
收稿时间:2019-08-29

Biochemical Components Evaluation and Optimal Harvest Period of the Leaves of Camellia Sect.Chrysantha
LIU Baocai,CHEN Jingying,ZHANG Wujun,HUANG Yingzhen,ZHAO Yunqing.Biochemical Components Evaluation and Optimal Harvest Period of the Leaves of Camellia Sect.Chrysantha[J].Chinese Journal of Tropical Crops,2021,42(1):159-167.
Authors:LIU Baocai  CHEN Jingying  ZHANG Wujun  HUANG Yingzhen  ZHAO Yunqing
Institution:1. Institute of Agricultural Bioresource, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China2. Research Center for Medicinal Plant, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
Abstract:26 accessions including six species of golden camellia,different phenotypes of Camellia chrysantha and C.chrysantha collected in different months were selected as the research materials.The simultaneous determination of rutin,quercetin,luteolin and kaempferiae by HPLC and the determination of tea polyphenols,flavonoids,total polysaccharides and total saponins by ultraviolet spectrophotometric were established and optimized.The quality of leaves of golden camellia from different sources was comprehensively evaluated based on the combination of the principal component comprehensive scores and clustering analysis of the eight biochemical components.There were differences in the content of the eight components in different sources.The content of the eight components in C.nitidissima was higher,followed by that of C.perpetua and C.chrysantha.The content of total saponins,total flavonoids and total polysaccharides in 8-year-old C.chrysantha collected from January to December all showed the rule of ascend ing-descending-ascending-descending,and reached the highest value in October.The order of the principal component comprehensive score of the 5-year-old different species(S1-S14)was as follows:C.nitidissima>C.perpetua>C.chrysantha(large flowers,many flowers,not cold-resistant,yellow flowers and large,sharp fruits)>C.euphlebi>C.impressinervis>C.nitidissima var.microcarpa.The order of the comprehensive scores of the main ingredients of the 8-year-old havested in different months(S15-S26)was October>February>March>April>May>July>June>January>August>December>November>September.Besides,the ranking of the comprehensive scores of large buds,large flowers,multi-golden flower and February to April and October harvested C.chrysantha was higher and at the first 10 ranks.Cluster analysis classified the 26 accessions could be classified into five categories.In summary,the quality of golden camellia was affected by variety,tree age and harvest time.The comprehensive biochemical compositions of C.nitidissima,C.perpetua and C.chrysantha with phenotype of large flower buds,many flowers,not cold resisdence,yellow and big flowers was better.It approved that the best harvest period of C.chrysantha was from February to April and October.The established multi-index quantitative analysis combined with chemometric analysis would provide a reference for biochemical evaluation of golden camellia.
Keywords:Camellia Sect  Chrysantha  simultaneous determination  chemometrics methods  quantitative evaluation  harvest period
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