| T-RFLP分析技术在肉鸡肠道微生物研究中的应用 |
| |
| 引用本文： | 张华,蔡辉益,刘国华,常文环,郝晓洁,周晓.T-RFLP分析技术在肉鸡肠道微生物研究中的应用[J].动物营养学报,2011,23(3):364-369. |
| |
| 作者姓名： | 张华 蔡辉益 刘国华 常文环 郝晓洁 周晓 |
| |
| 作者单位： | 中国农业科学院饲料研究所,家禽营养与饲料研究室,北京,100081 |
| |
| 基金项目： | 国家肉鸡产业技术体系;中央级公益性科研院所基本科研业务费专项 |
| |
| 摘 要： | 本文阐述了末端限制性片段长度多态性(T-RFLP)分析技术的基本原理和技术路线,讨论了应用T-RFLP分析技术相对于应用传统方法研究肉鸡肠道微生物的优势,总结了应用T-RFLP分析技术研究肉鸡肠道微生物的发展历程,分析了应用T-RFLP分析技术研究肉鸡肠道微生物存在的问题及解决方法,并对其应用前景进行了展望.
|
| 关 键 词： | T-RFLP分析技术 肉鸡 肠道微生物 |
Application of T-RFLP Technique in the Studying of Gut Microflora in Broilers |
| |
| ZHANG Hua,CAI Huiyi,LIU Guohua,CHANG Wenhuan,HAO Xiaojie,ZHOU Xiao.Application of T-RFLP Technique in the Studying of Gut Microflora in Broilers[J].Acta Zoonutrimenta Sinica,2011,23(3):364-369. |
| |
| Authors: | ZHANG Hua CAI Huiyi LIU Guohua CHANG Wenhuan HAO Xiaojie ZHOU Xiao |
| |
| Institution: | ZHANG Hua CAI Huiyi LIU Guohua CHANG Wenhuan HAO Xiaojie ZHOU Xiao(Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
| |
| Abstract: | The basic principles and technical route of T-RFLP(terminal-restriction fragment length polymorphism)were introduced in this paper,the advantages of T-RFLP over traditional research methods for studying intestinal microflora of broilers were discussed.Meanwhile,the development of the application of T-RFLP technique for studying microflora in the broiler gut and its problems and solutions were also summarized in this paper. |
| |
| Keywords: | T-RFLP technique broiler gut microflora |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
