| 协同指数法筛选银翘天甘组方的最佳剂量配比 |
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| 引用本文： | 王宏军,周铁忠,刘孝刚,王金莉,刘月.协同指数法筛选银翘天甘组方的最佳剂量配比[J].动物医学进展,2011,32(1):58-62. |
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| 作者姓名： | 王宏军 周铁忠 刘孝刚 王金莉 刘月 |
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| 作者单位： | 辽宁医学院,辽宁锦州,121001 |
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| 基金项目： | 辽宁省教育厅资助项目，辽宁省财政厅资助项目 |
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| 摘 要： | 为了研究银翘天甘组方中金银花、连翘、天花粉、甘草4味中药的最佳剂量配比,利用SI测定方法设计试验,微管-平板法测定各试验药物的MIC,计算SI值,依据呈现协同作用的SI值获得组方剂量配比,以浸膏率与MIC为验证依据,确定组方的最佳剂量配比.结果表明,四味中药配伍体外抗菌活性呈现协同作用的SI值在0.625～0.750之...
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| 关 键 词： | 中药 协同指数 最小抑菌浓度 |
Screening Optimal Dose Ratio of Yinqiaotiangan Compound by Method of Synergic Index |
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| WANG Hong-jun,ZHOU Tie-zhong,LIU Xiao-gang,WANG Jin-li,LIU Yue.Screening Optimal Dose Ratio of Yinqiaotiangan Compound by Method of Synergic Index[J].Progress In Veterinary Medicine,2011,32(1):58-62. |
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| Authors: | WANG Hong-jun ZHOU Tie-zhong LIU Xiao-gang WANG Jin-li LIU Yue |
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| Institution: | (Liaoning Medical University,Jinzhou,Liaoning,121001,China) |
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| Abstract: | In order to study on the optimal dose ratio of Flos loniceraer,Forsythia fruit,Radix trichosanthis and Glycyrrhizae radixt in Yinqiaotiangan compound,the test was designed by the assay method of the synergic index(SI).The minimal inhibition concentration(MIC) of every drug was determined by the microbule-plate methods.SI was calculated by the formula.The dose matching of four Chinese herbs would come from SI of presenting synergism.Results showed that the SI of presenting synergism was 0.625-0.750.The results of MIC showed that the matching of Flos loniceraer,Forsythia fruit,Radix trichosanthis and Glycyrrhizae radixt was 2∶3∶9∶4 which presented the better antibacterial effect on E.coli in vitro.The results of MIC showed that the matching of them was 1∶6∶72∶2 which presented good antibacterial effect on Streptococcus suis Ⅱ in vitro.The results of verification test showed that extracted rate of the former was significantly higher than that of the latter(P<0.05).The antibacterial effect of the former was significantly higher than that of the latter(P<0.01) on E.coli and antibacterial effect of the former was better than that of the latter on Streptococcus suis Ⅱ in vitro.MIC of the former was 20 μg/mL to E.coli and 30 μg/mL to Streptococcus suis Ⅱ.So the optimal matching of Flos loniceraer,Forsythia fruit,Radix trichosanthis and Glycyrrhizae radixt in Yinqiaotiangan compound was 2∶3∶9∶4. |
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| Keywords: | traditional Chinese medicine synergic index minimal inhibition concentration |
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