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Development of a formulation of Trichoderma asperellum to control black rot disease on pineapple caused by (Thielaviopsis paradoxa) |
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| Authors: | CJ Wijesinghe RS Wilson WijeratnamJKRR Samarasekara RLC Wijesundera |
| Institution: | a Post Harvest Technology Laboratory, Food Technology, Industrial Technology Institute, 363, Bauddhaloka Mawatha, Colombo 07, Sri Lanka b Herbal Technology Laboratory, Industrial Technology Institute, 363, Bauddhaloka Mawatha, Colombo 07, Sri Lanka c Department of Plant Sciences, University of Colombo, Colombo 03, Sri Lanka |
| Abstract: | A local isolate of Trichoderma asperellum was tested for its antagonistic activity against Thielaviopsis paradoxa (telemorph = Ceratocyctis paradoxa). The highest antagonistic activity was achieved when the concentration of T. asperellum conidia was 1 × 107 conidia/mL. The highest biomass and number of colony forming unit/mL of the T. asperellum peaked at 144 h after incubation in yeast waste residue medium. The minimum inhibition concentration value of the formulation was observed as 1% on growth of Th. paradoxa incubated at 28 ± 2 °C for 10 d. In the soil fungicide-screening test, the effect of concentrations 100-1600 μg/mL on mycelia growth was not significant (P < 0.05). Complete mycelial growth inhibition occurred at concentration above 52,600 μg/mL. Results of the fruit application tests clearly showed that all treated fruits were free of disease at the end of the incubation period. No significant differences (P > 0.05) in pH, total soluble solids and titratable acidity were observed between fruits treated with formulation of T. asperellum and the control formulation treated pineapples. |
| Keywords: | Trichoderma asperellum Biocontrol formulation Yeast waste residue Pineapple black rot disease |
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